Zhao Zhen-Zhu, Zhang Fei, Ji Bao-Yu, Zhou Ning, Chen Hui, Sun Yan-Jun, Feng Wei-Sheng, Zheng Xiao-Ke
School of Pharmacy, Henan University of Chinese Medicine Zhengzhou 450046 China
RSC Adv. 2023 Jun 16;13(27):18223-18228. doi: 10.1039/d3ra02672h. eCollection 2023 Jun 15.
Nine pyrrole alkaloid derivatives, including four new ones (1-4), were isolated from the wild mushroom for the first time. Their chemical structures were determined using UV-Vis spectroscopy, IR spectroscopy, MS, NMR spectroscopy, and single-crystal X-ray diffraction techniques. Compound 1, a previously unreported bicylo-pyrrole aldehyde homologue, was found to be a major component, approximately 8.2 μg g in the dry powder of . Compound 1 showed cytotoxicity against SMMC-772 (IC 15.8 μM) without any cytotoxic effect on LO2, a normal hepatic cell line; compounds 1 and 2 displayed weak immunosuppressive activities by inhibiting the proliferation of induced T cells; compound 3 showed inhibition activity on the proliferation of HaCaT cell line (IC 25.4 μM) and weak antioxidant activity at a concentration of 50 μM.
首次从该野生蘑菇中分离出9种吡咯生物碱衍生物，其中包括4种新的衍生物（1-4）。使用紫外-可见光谱、红外光谱、质谱、核磁共振光谱和单晶X射线衍射技术确定了它们的化学结构。化合物1是一种以前未报道的双环吡咯醛同系物，被发现是主要成分，在其干粉中约为8.2μg/g。化合物1对SMMC-772显示出细胞毒性（IC50为15.8μM），而对正常肝细胞系LO2没有任何细胞毒性作用；化合物1和2通过抑制诱导T细胞的增殖表现出弱的免疫抑制活性；化合物3对HaCaT细胞系的增殖显示出抑制活性（IC50为25.4μM），并且在浓度为50μM时表现出弱的抗氧化活性。
