State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Key Laboratory of Genetic Network Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
Genome Biol. 2020 Mar 23;21(1):66. doi: 10.1186/s13059-020-01966-9.
Many differential gene expression analyses are conducted with an inadequate number of biological replicates. We describe an easy and effective RNA-seq approach using molecular barcoding to enable profiling of a large number of replicates simultaneously. This approach significantly improves the performance of differential gene expression analysis. Using this approach in medaka (Oryzias latipes), we discover novel genes with sexually dimorphic expression and genes necessary for germ cell development. Our results also demonstrate why the common practice of using only three replicates in differential gene expression analysis should be abandoned.
许多差异基因表达分析的生物学重复数不足。我们描述了一种简单有效的 RNA-seq 方法，使用分子条形码来同时对大量重复样本进行分析。这种方法显著提高了差异基因表达分析的性能。我们在斑马鱼（Oryzias latipes）中使用这种方法，发现了具有性别二态性表达的新基因和生殖细胞发育所必需的基因。我们的结果还表明，为什么应该摒弃在差异基因表达分析中仅使用三个重复的常见做法。
