Faraji Darab, Ebrahimi Mohsen, Paknezhad Babak, Hami Zahra, Jahandideh Alireza
Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Department of Toxicology and Pharmacology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Bull Emerg Trauma. 2020 Jan;8(1):10-18. doi: 10.29252/beat-080103.
To assess regenerative capacities of chitosan-nanoselenium conduit on transected sciatic nerve in diabetic rats.
A 10-mm sciatic nerve defect was bridged using a chitosan-nanoselenium conduit filled with phosphate buffered saline. In chitosan group, the chitosan conduit was filled with phosphate buffered saline solution. In sham-operated group, sciatic nerve was exposed and closed. In transected group, right sciatic nerve was transected and nerve cut ends were fixed in the adjacent muscle. The regenerated fibers were studied within 12 weeks after surgery.
The behavioral and functional and electrophysiological tests confirmed faster recovery of the regenerated axons in chitosan-nanoselenium conduit group compared to chitosan group (0.001). The mean ratios of gastrocnemius muscles weight were measured. There was statistically significant difference between the muscle weight ratios of chitosan-nanoselenium conduit and chitosan groups (0.001). Morphometric indices of regenerated fibers showed number and diameter of the myelinated fibers were significantly higher in chitosan-nanoselenium conduit group than in chitosan group.
chitosan-nanoselenium conduit resulted in acceleration of functional recovery and quantitative morphometric indices of sciatic nerve.
评估壳聚糖-纳米硒导管对糖尿病大鼠坐骨神经横断伤的再生能力。
使用填充有磷酸盐缓冲盐水的壳聚糖-纳米硒导管桥接10毫米的坐骨神经缺损。壳聚糖组中，壳聚糖导管填充磷酸盐缓冲盐溶液。假手术组中，暴露并闭合坐骨神经。横断组中，切断右侧坐骨神经并将神经断端固定于相邻肌肉。术后12周内研究再生纤维。
行为、功能和电生理测试证实，与壳聚糖组相比，壳聚糖-纳米硒导管组再生轴突恢复更快（P<0.001）。测量腓肠肌重量的平均比值。壳聚糖-纳米硒导管组与壳聚糖组的肌肉重量比值存在统计学显著差异（P<0.001）。再生纤维的形态学指标显示，壳聚糖-纳米硒导管组有髓纤维的数量和直径显著高于壳聚糖组。
壳聚糖-纳米硒导管可加速坐骨神经功能恢复及定量形态学指标改善。
