University Hospital Tübingen, Section Medical Materials Science & Technology, Osianderstr. 2-8, Tübingen D-72076, Germany.
University Hospital Tübingen, Department of Thoracic, Cardiac and Vascular Surgery, Calwerstr. 7/1, D-72076 Tübingen, Germany.
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110701. doi: 10.1016/j.msec.2020.110701. Epub 2020 Jan 28.
Peri-implantitis is the most important issue threatening the long-term survival rate of dental implants. Various efforts have been made to reduce implant surface plaque formation, which is one of the essential causes of peri-implantitis. In our study, we applied the natural antibacterial agent totarol as a coating on experimental silicon wafer and titanium implant surfaces. To analyze the interaction between the totarol coating and the oral primary colonizer S. gordonii and isolates of mixed oral bacteria, samples were incubated in a model system simulating the oral environment and analyzed by Live/Dead staining, crystal violet staining and scanning electron microscopy (SEM). After 4 d, 8 d, 12 d, 16 d, and 24 d salivary incubation, the stability and antibacterial efficiency of totarol coating was evaluated through SEM. The results indicated that totarol coatings on both silicon wafer and Ti surfaces caused efficient contact killing and an inhibition effect towards S. gordonii and mixed oral bacterial film growth after 4 h, 8 h, 24 h, and 48 h incubation. After longtime salivary incubation of 12 d, the bactericidal effect started to weaken, but the anti-adhesion and inhibition effect to biofilm development still exist after 24 d of salivary incubation. The application of a totarol coating on implant or abutment surfaces is a promising potential prophylactic approach against peri-implantitis.
种植体周围炎是威胁种植牙长期存活率的最重要问题。人们已经做出了各种努力来减少种植体表面牙菌斑的形成，这是种植体周围炎的一个重要原因。在我们的研究中，我们将天然抗菌剂松萝酸作为涂层应用于实验硅片和钛种植体表面。为了分析松萝酸涂层与口腔主要定植菌 S. gordonii 和混合口腔细菌分离株之间的相互作用，将样品在模拟口腔环境的模型系统中孵育，并通过 Live/Dead 染色、结晶紫染色和扫描电子显微镜（SEM）进行分析。在唾液孵育 4 d、8 d、12 d、16 d 和 24 d 后，通过 SEM 评估松萝酸涂层的稳定性和抗菌效率。结果表明，松萝酸涂层在硅片和 Ti 表面上，在孵育 4 h、8 h、24 h 和 48 h 后，对 S. gordonii 和混合口腔细菌薄膜生长均具有高效的接触杀伤和抑制作用。经过 12 d 的长时间唾液孵育后，杀菌效果开始减弱，但在唾液孵育 24 d 后，仍存在抗黏附和抑制生物膜发展的作用。在种植体或基台表面应用松萝酸涂层是预防种植体周围炎的一种很有前途的潜在方法。
