Department of Biotechnology and Medical Engineering, National Institute of Technology Rourkela, Rourkela, Odisha, India.
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal, India.
Mater Sci Eng C Mater Biol Appl. 2020 May;110:110647. doi: 10.1016/j.msec.2020.110647. Epub 2020 Jan 7.
Evaluation of the biological properties of silanized graphene oxide is important in the context of biomedical applications of the material. In this study, we have evaluated the toxicity, immunogenicity and other biological properties like osteogenicity of silanized graphene oxide (SiGO). Graphene oxide (GO) was silanized using a common silanizing agent namely (3-aminopropyl) triethoxysilane (APTES). Silanization was confirmed through infrared spectroscopy and elemental mapping. Post-silanization, we did not observe any significant changes in the morphology of GO. Silanization leads to an increase in the interlayer distance and disorder in the lattice. Study of in vitro toxicity of SiGO on three different cell lines namely primary human dermal fibroblast, murine embryonic fibroblast and human osteosarcoma cell lines revealed that toxicity of SiGO was significantly less than GO. We further showed that in vitro immune activation of macrophage was less in the case of SiGO in comparison to GO. Profiling of osteogenic differentiation of human mesenchymal stem cell revealed that SiGO is less osteogenic than GO. Study of acute toxicity in the murine model indicated that GO was hepatotoxic at experimental concentration whereas SiGO did not show any significant toxicity. This study implied that SiGO is a better biocompatible material than GO.
评估硅烷化氧化石墨烯的生物学特性对于该材料在生物医学应用中的重要性。在本研究中，我们评估了硅烷化氧化石墨烯（SiGO）的毒性、免疫原性和其他生物学特性，如成骨性。氧化石墨烯（GO）使用常见的硅烷化剂（3-氨丙基三乙氧基硅烷（APTES））进行硅烷化。通过红外光谱和元素映射证实了硅烷化。硅烷化后，我们没有观察到 GO 形态有任何明显变化。硅烷化导致层间距增加和晶格无序。研究 SiGO 对三种不同细胞系（原代人真皮成纤维细胞、鼠胚胎成纤维细胞和人骨肉瘤细胞系）的体外毒性，结果表明 SiGO 的毒性明显小于 GO。我们进一步表明，与 GO 相比，SiGO 体外激活巨噬细胞的免疫活性较低。人骨髓间充质干细胞成骨分化分析表明，SiGO 的成骨性低于 GO。在小鼠模型中研究急性毒性表明，GO 在实验浓度下具有肝毒性，而 SiGO 没有显示出任何显著的毒性。这项研究表明，SiGO 比 GO 具有更好的生物相容性。
