Grecca Igor Soares Gianini, Miola Vitor Fernando Bordin, Ferreira Júlia Carolina, Vinholo Thiago Rissato, da Silva Laira Mireli Dias, Totti Paulo Gabriel Friedrich, Gianini Silvia Helena Soares, Souza Maricelma da Silva Soares de, Souza Juliana da Silva Soares de, Araújo Adriano Cressoni, Guiguer Elen Landgraf, Spilla Caio Sérgio Galina, Bechara Marcelo Dib, Roque Domingos Donizeti, Pereira Eliana de Souza Bastos Mazuqueli, Pomini Karina Torres
School of Medicine, University of Marília (UNIMAR), Avenida Hygino Muzzy Filho, Marília 17525-902, SP, Brazil.
Department of Periodontics, School of Medicine, University of Marília (UNIMAR), Avenida Hygino Muzzy Filho, Marília 17525-902, SP, Brazil.
Pharmaceutics. 2025 Aug 21;17(8):1088. doi: 10.3390/pharmaceutics17081088.
Mesenchymal stem cells (MSCs) represent a promising strategy in the field of regenerative medicine due to their multipotent differentiation capacity and immunomodulatory properties. The interaction of these cells with the extracellular matrix (ECM) and biomaterials, notably graphene oxide (GO), has proven decisive in modulating cell behavior, with the potential to optimize tissue regeneration processes. This review was conducted using the MEDLINE, Scopus, and Cochrane databases, covering studies published between 2018 and 2025, from which seven studies met the inclusion criteria, with an emphasis on in vitro and in vivo investigations regarding the association between GO and MSCs. The main findings demonstrate that GO, particularly when conjugated with polymers such as poly(L-lactic acid) (PLLA), enhances cell adhesion, stimulates proliferation, and promotes the osteogenic differentiation of MSCs, in addition to positively modulating intracellular signaling pathways. However, significant gaps remain in understanding the mechanisms and safety of GO's therapeutic use in association with MSCs. Therefore, this review reinforces the need for further studies to deepen the characterization of the bioactive properties of GO-MSCs, aiming to enable safer and more effective clinical applications.
间充质干细胞（MSCs）由于其多能分化能力和免疫调节特性，在再生医学领域代表着一种有前景的策略。这些细胞与细胞外基质（ECM）和生物材料，特别是氧化石墨烯（GO）的相互作用，已被证明在调节细胞行为方面起决定性作用，具有优化组织再生过程的潜力。本综述使用MEDLINE、Scopus和Cochrane数据库进行，涵盖2018年至2025年发表的研究，其中七项研究符合纳入标准，重点是关于GO与MSCs之间关联的体外和体内研究。主要研究结果表明，GO，特别是当与聚（L-乳酸）（PLLA）等聚合物共轭时，除了正向调节细胞内信号通路外，还能增强细胞粘附、刺激增殖并促进MSCs的成骨分化。然而，在理解GO与MSCs联合治疗应用的机制和安全性方面仍存在重大差距。因此，本综述强调需要进一步研究以深化对GO-MSCs生物活性特性的表征，旨在实现更安全、更有效的临床应用。
