State Key Laboratory for Oncogenes and Related Genes, Division of Cardiology, Renji Hospital, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
State Key Laboratory for Oncogenes and Related Genes, Division of Cardiology, Renji Hospital, Shanghai Cancer Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
J Mol Cell Cardiol. 2020 May;142:80-92. doi: 10.1016/j.yjmcc.2020.03.005. Epub 2020 Mar 20.
Single-cell RNA sequencing (scRNA-seq), a method of transcriptome sequencing at the single-cell level, has recently emerged as a revolutionary technology in the field of biomedical research. Compared to conventional gene expression profiling in bulk, scRNA-seq resolves biological differences among individual cells and enables the identification of rare cell populations that are easily overlooked. This review introduces the method of scRNA-seq, summarizes its applications in the field of cardiovascular disease research, and discusses existing limitations and prospects for future applications.
单细胞 RNA 测序（scRNA-seq）是一种在单细胞水平上进行转录组测序的方法，最近在生物医学研究领域成为一项革命性技术。与批量进行的常规基因表达谱分析相比，scRNA-seq 能够解析单个细胞之间的生物学差异，并能够识别容易被忽视的稀有细胞群体。本文介绍了 scRNA-seq 的方法，总结了其在心血管疾病研究领域的应用，并讨论了其存在的局限性和未来应用的前景。
