ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, University of Wollongong, Wollongong, NSW, Australia.
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.
Methods Mol Biol. 2020;2140:159-170. doi: 10.1007/978-1-0716-0520-2_10.
Bioprinting cells with an electrically conductive bioink provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the option of electrically stimulating cells in situ during and after tissue development. We and others have demonstrated the use of electrical stimulation (ES) to influence cell behavior and function for a more biomimetic approach to tissue engineering. Here, we detail a previously published method for 3D printing an electrically conductive bioink with human neural stem cells (hNSCs) that are subsequently differentiated. The differentiated tissue constructs comprise functional neurons and supporting neuroglia and are amenable to ES for the purposeful modulation of neural activity. Importantly, the method could be adapted to fabricate and stimulate neural and nonneural tissues from other cell types, with the potential to be applied for both research- and clinical-product development.
使用具有导电性的生物墨水打印细胞为原位组织发育过程中及之后对细胞进行电刺激提供了一个机会，从而可以生成具有三维（3D）结构的细胞载体结构。我们和其他人已经证明了电刺激（ES）在影响细胞行为和功能方面的作用，这为更仿生的组织工程方法提供了思路。在这里，我们详细介绍了一种之前发表的方法，用于 3D 打印具有人神经干细胞（hNSC）的导电性生物墨水，随后对其进行分化。分化后的组织构建体包含功能性神经元和支持性神经胶质细胞，并且可以进行 ES 处理，以有针对性地调节神经活动。重要的是，该方法可以适应于制造和刺激来自其他细胞类型的神经组织和非神经组织，具有应用于研究和临床产品开发的潜力。
