三维生物打印人诱导多能干细胞构建体用于多谱系组织工程和建模。
Bioprinting 3D Human Induced Pluripotent Stem Cell Constructs for Multilineage Tissue Engineering and Modeling.
机构信息
ARC Centre of Excellence for Electromaterials Science, Intelligent Polymer Research Institute, AIIM Facility, University of Wollongong, Wollongong, NSW, Australia.
Illawarra Health and Medical Research Institute, University of Wollongong, Wollongong, NSW, Australia.
出版信息
Methods Mol Biol. 2020;2140:251-258. doi: 10.1007/978-1-0716-0520-2_17.
Bioprinting human pluripotent stem cells (PSCs) provides an opportunity to produce three-dimensional (3D) cell-laden constructs with the potential to be differentiated in vitro to all tissue types of the human body. Here, we detail a previously published method for 3D printing human induced pluripotent stem cells (iPSCs; also applicable to human embryonic stem cells) within a clinically amenable bioink (also described in Chapter 10 ) that is cross-linked to a 3D construct. The printed iPSCs continue to have self-replicating and multilineage cell induction potential in situ, and the constructs are robust and amenable to different differentiation protocols for fabricating diverse tissue types, with the potential to be applied for both research- and clinical-product development.
生物打印人类多能干细胞（PSCs）为体外分化为人体所有组织类型提供了一种生产具有细胞负载的三维（3D）构建体的机会。在这里，我们详细介绍了一种先前发表的方法，用于在临床可用的生物墨水中（也在第 10 章中描述）打印 3D 人类诱导多能干细胞（iPSCs；也适用于人类胚胎干细胞），该墨水可交联到 3D 构建体中。打印的 iPSCs 在原位继续具有自我复制和多能性细胞诱导潜力，并且构建体坚固且可适用于不同的分化方案，用于制造多种组织类型，具有应用于研究和临床产品开发的潜力。
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