Martínez Leo Edwin E, Segura Campos Maira R
Facultad de Ingeniería Quimica, Universidad Autonoma de Yucatan, Mérida, Yucatán, México.
J Food Biochem. 2020 Jun;44(6):e13207. doi: 10.1111/jfbc.13207. Epub 2020 Mar 24.
Neuroinflammation plays a critical role in the neurodegenerative disease's development, where microglia's act an important role in the mechanisms of response to neuronal damage. In the present research, the neuroprotective effect from Salvia hispanica peptide fractions on the proinflammatory modulation on HMC3 microglial cells was evaluated. From the enzymatic hydrolysis of a protein-rich fraction from S. hispanica seeds, three peptide fractions (<1, 1-3 and 3-5 kDa) were obtained, from which its neuroprotective and anti-inflammatory effect was determined on the production of proinflammatory mediators on HMC3 cells. The F1-3 kDa exhibited the greatest protective effect (79.04%), associated with the decrease in ROS cell production (51.3 ± 2.3%). Likewise, F1-3 kDa at 50 µg/ml, presented the highest reduction percentages of NO (33.1 ± 2.30%), TNFα (26.4 ± 1.1%) and IL6 (17.36 ± 1.6%). F1-3 kDa exhibited a neuroprotective effect in HMC3 cells associated with its antioxidant and anti-inflammatory effect. PRACTICAL APPLICATIONS: Currently, neurodegenerative diseases represent a global health problem, so the search for bioactive compounds with neuroprotective effect is useful in the prevention and treatment of this group of diseases. Peptide research with an effect on the proinflammatory and prooxidant mediator's reduction presents a potential application in the functional food's development aimed at the treatment of chronic diseases, that have oxidative stress and inflammation as their etiological factor. The present research adds to the scientific evidence of the potential benefits of bioactive peptides obtained from chia seeds. The results correlate with the main health benefits of whole chia seed in humans, such as antioxidant, anti-inflammatory, hypoglycemic and hypotensive capacity. This relationship is associated with the protein and peptide composition of chia, which increases its added value as food.
神经炎症在神经退行性疾病的发展中起关键作用，其中小胶质细胞在对神经元损伤的反应机制中起重要作用。在本研究中，评估了西班牙鼠尾草肽组分对HMC3小胶质细胞促炎调节的神经保护作用。通过对西班牙鼠尾草种子中富含蛋白质的部分进行酶解，获得了三个肽组分（<1、1 - 3和3 - 5 kDa），并测定了它们对HMC3细胞中促炎介质产生的神经保护和抗炎作用。1 - 3 kDa的组分表现出最大的保护作用（79.04%），这与细胞内活性氧生成的减少（51.3 ± 2.3%）相关。同样，50 μg/ml的1 - 3 kDa组分对一氧化氮（33.1 ± 2.30%）、肿瘤坏死因子α（26.4 ± 1.1%）和白细胞介素6（17.36 ± 1.6%）的降低百分比最高。1 - 3 kDa组分在HMC3细胞中表现出神经保护作用，与其抗氧化和抗炎作用相关。实际应用：目前，神经退行性疾病是一个全球性的健康问题，因此寻找具有神经保护作用的生物活性化合物对于预防和治疗这类疾病是有用的。对促炎和促氧化介质减少有作用的肽研究在旨在治疗以氧化应激和炎症为病因的慢性疾病的功能性食品开发中具有潜在应用价值。本研究增加了从奇亚籽中获得的生物活性肽潜在益处的科学证据。这些结果与奇亚籽对人体的主要健康益处相关，如抗氧化、抗炎、降血糖和降血压能力。这种关系与奇亚籽的蛋白质和肽组成有关，这增加了其作为食品的附加值。
