Facultad de Ingenieria Quimica, Universidad Autonoma de Yucatan, Periférico Norte Km, 33.5, Tablaje Catastral 13615, Col. Chuburná de Hidalgo Inn, 97203, Merida, Yucatan, Mexico.
Neurochem Res. 2020 Oct;45(10):2278-2285. doi: 10.1007/s11064-020-03085-0. Epub 2020 Jul 16.
Neurodegenerative diseases (ND) affect around a billion people worldwide. Oxidative stress plays a critical role in the activation of neuronal death mechanisms, implicated in the ND etiology. In the present research, the neuroprotective effect of the S. hispanica protein derivatives is evaluated, on neuronal cells N1E-115, after the damage induction with HO. From the protein-rich fraction of S. hispanica, three peptide fractions were obtained (3-5, 1-3 y < 1 kDa) and its neuroprotective effect on neuronal cells N1E-115 was evaluated, through the antioxidant pathway. In the toxicity assay, the peptide fractions showed viability greater than 90%. When N1E-115 cells were incubated with 100 µM HO, fractions 1-3 and < 1 kDa, presented cell viability of 66.64% ± 3.2 and 67.32% ± 2.8, respectively. Fractions 1-3 and < 1 kDa reduced by 41.73% ± 3.2 and 40.87% ± 2.8, respectively, the ROS production compared to the control, without significant statistical difference between both fractions (p < 0.05), while F3-5 kDa, only reduced the ROS production by 21.95% ± 2.4. The protective effect observed in the < 3 kDa fractions could be associated with its antioxidant activity, which represents an important study target.
神经退行性疾病（ND）影响全球约 10 亿人。氧化应激在神经元死亡机制的激活中起着关键作用，与 ND 的病因有关。在本研究中，评估了 S. hispanica 蛋白衍生物对 N1E-115 神经元细胞的神经保护作用，该细胞在 HO 诱导损伤后。从 S. hispanica 的富含蛋白质部分获得了三个肽部分（3-5、1-3 和 <1 kDa），并通过抗氧化途径评估了其对 N1E-115 神经元细胞的神经保护作用。在毒性测定中，肽部分的活力大于 90%。当 N1E-115 细胞用 100 µM HO 孵育时，第 1-3 部分和 <1 kDa 部分的细胞活力分别为 66.64%±3.2 和 67.32%±2.8。第 1-3 部分和 <1 kDa 部分分别减少了 41.73%±3.2 和 40.87%±2.8 的 ROS 产生，与对照组相比，没有显著统计学差异（p<0.05），而 F3-5 kDa 仅减少了 21.95%±2.4 的 ROS 产生。在 <3 kDa 部分观察到的保护作用可能与其抗氧化活性有关，这是一个重要的研究目标。
