Department of Neurology, the Second Hospital of Dalian Medical University, Dalian City, China.
Department of Endocrinology, the Second Hospital of Dalian Medical University, Dalian City, China.
Cell Reprogram. 2020 Jun;22(3):147-155. doi: 10.1089/cell.2019.0088. Epub 2020 Mar 24.
Complications of central nervous system in type 2 diabetes mellitus (T2DM) often lead to cognitive impairment and seriously affect the quality of life. However, there is no individualized disease model. Urine-derived stem cells can be an ideal source for generating human induced pluripotent stem cells (hiPSCs) and progenitors, as they are easily accessible, noninvasive, and universally available. In our research, we differentiated urine-derived hiPSCs into neuron (N), astrocyte (A), and microvascular endothelial cells (E) from a T2DM patient. Next step, we intend to coculture these three cells together in a 3D system to create a new disease model , which may simulate the cerebral microenvironment of DM, for future study of its pathogenesis and precision medical treatment.
2 型糖尿病（T2DM）患者中枢神经系统并发症常导致认知障碍，严重影响生活质量。然而，目前尚无个体化疾病模型。尿源干细胞可作为生成人类诱导多能干细胞（hiPSC）及其前体细胞的理想来源，因为它们易于获取、非侵入性且普遍存在。在我们的研究中，我们从 T2DM 患者的尿液中分离出 hiPSC，并将其分化为神经元（N）、星形胶质细胞（A）和微血管内皮细胞（E）。下一步，我们打算将这三种细胞在 3D 系统中共培养，以创建一种新的疾病模型，模拟 DM 的大脑微环境，用于未来研究其发病机制和精准医疗。
