血清样本的酸水解以提高HIV抗原的检测率。
Acid hydrolysis of serum samples to increase detection of HIV antigen.
作者信息
Mathiesen T, Sundqvist V A, Albert J, Ohlsson E, Wahren B
机构信息
Department of Virology, National Bacteriological Laboratory, Stockholm, Sweden.
出版信息
J Virol Methods. 1988 Dec;22(2-3):143-8. doi: 10.1016/0166-0934(88)90097-3.
Sera from 32 HIV-infected patients and 20 controls were assayed for HIV antigen (HIV-Ag) and antibodies following acid hydrolysis. Acid hydrolysis, followed by neutralization immediately prior to the assay, was found to be a simple means to solubilize immune complexes and allowed recovery of 40-50% of complexed antigen. Following acid hydrolysis, HIV-Ag levels rose or became detectable in 22/32 patients and HIV IgG1-4 levels rose in 17/20 patients studied. The results show that complexed HIV-Ag may evade detection in HIV-infected patients.
对32名HIV感染患者和20名对照者的血清进行酸水解后检测HIV抗原（HIV-Ag）和抗体。酸水解后，在检测前立即中和，被发现是溶解免疫复合物的一种简单方法，可回收40%-50%的复合抗原。酸水解后，22/32的患者HIV-Ag水平升高或变得可检测，在17/20的研究患者中HIV IgG1-4水平升高。结果表明，复合HIV-Ag可能在HIV感染患者中逃避检测。
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