1型人类免疫缺陷病毒游离抗原和复合抗原在4℃及室温下的稳定性。
Stability of free and complexed human immunodeficiency virus type 1 antigen at 4 degrees C and at room temperature.
作者信息
Griffith B P, Garner R B, Chacko T M
机构信息
Retrovirus Diagnostic Section, National Virology Reference Laboratory, VA Medical Center, West Haven, Connecticut 06516, USA.
出版信息
J Clin Microbiol. 1995 May;33(5):1348-50. doi: 10.1128/jcm.33.5.1348-1350.1995.
Abstract
Free and immune-complex-dissociated (ICD) human immunodeficiency virus type 1 (HIV-1) antigenemias in serum specimens stored at room temperature (RT) and 4 degrees C for 1 to 35 days were evaluated. At all time points examined, there was no significant loss in detectable levels of ICD HIV-1 antigen at either RT or 4 degrees C. Free HIV-1 antigen was not stable in serum samples stored at RT for more than 2 days but was stable in samples stored at 4 degrees C for up to 4 days. Loss of free antigen occurred more rapidly in samples with high antigen content at baseline. Use of the ICD antigen assay allowed accurate quantitation of antigen in samples stored at RT or 4 degrees C for as long as 1 month.
摘要
对在室温（RT）和4℃下储存1至35天的血清标本中游离和免疫复合物解离（ICD）的1型人类免疫缺陷病毒（HIV-1）抗原血症进行了评估。在所有检测的时间点，RT或4℃下ICD HIV-1抗原的可检测水平均无显著损失。游离HIV-1抗原在RT储存超过2天的血清样本中不稳定，但在4℃储存长达4天的样本中稳定。基线时抗原含量高的样本中游离抗原的损失更快。使用ICD抗原检测法可对在RT或4℃下储存长达1个月的样本中的抗原进行准确定量。
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