一种用于检测携带者血液中HIV抗原的改进方法。
An improved method for the detection of HIV antigen in the blood of carriers.
作者信息
Kageyama S, Yamada O, Mohammad S S, Hama S, Hattori N, Asanaka M, Nakayama E, Matsumoto T, Higuchi F, Kawatani T
机构信息
Department of Virology, Tottori University School of Medicine, Yonago, Japan.
出版信息
J Virol Methods. 1988 Dec;22(2-3):125-31. doi: 10.1016/0166-0934(88)90095-x.
The measurement of HIV antigen levels in sera or plasma of HIV-infected individuals is critical for determining the existence of antigen or infectious virus before seroconversion and for prognosis. Pretreatment of sera or plasma of HIV carriers by heating at 70 degrees C for 10 min at an acidic pH enabled us to estimate antigens efficiently in immune complexes. This procedure will also be useful in determining antigen levels in HIV carriers more precisely.
检测HIV感染者血清或血浆中的HIV抗原水平对于确定血清转化前抗原或传染性病毒的存在以及预后至关重要。通过在酸性pH条件下70摄氏度加热10分钟对HIV携带者的血清或血浆进行预处理，使我们能够有效地估计免疫复合物中的抗原。该程序在更精确地确定HIV携带者的抗原水平方面也将有用。
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