Hsa_circ_0008792的上调通过调控Hsa-miR-711/ZFP1抑制骨肉瘤细胞的侵袭和迁移并促进细胞凋亡。
Up-Regulation of Hsa_circ_0008792 Inhibits Osteosarcoma Cell Invasion and Migration and Promotes Apoptosis by Regulating Hsa-miR-711/ZFP1.
作者信息
Chen Lu, Shan Yu, Zhang Hengshuo, Wang Hongliang, Chen Yunzhen
机构信息
Department of Spine Surgery, Qilu Hospital, Shandong University, Jinan, Shandong Province 250012, Republic of China.
出版信息
Onco Targets Ther. 2020 Mar 11;13:2173-2181. doi: 10.2147/OTT.S239256. eCollection 2020.
OBJECTIVE
Circular RNA is a newly discovered non-coding RNA. It plays an important role in regulating gene expression, and may take part in tumor progression. This study aimed to investigate the functions of hsa_circ_0008792 in osteosarcoma regulation.
METHODS
We identified a circular RNA, hsa_circ_0008792, by using bioinformatics to analyze the GSE96962 dataset. The capacities of migration and invasion were assessed by wound-healing assay and transwell Matrigel assay. The ratios of G0/G1, S, and G2/M phases in cell cycle and apoptosis were measured using flow cytometry.
RESULTS
Hsa_circ_0008792 is expressed at low levels in osteosarcoma cells, and up-regulation of hsa_circ_0008792 could suppress osteosarcoma cell migration and invasion and promote apoptosis. This regulation is mediated by hsa-miR-711/ZFP1. The expression level of hsa_circ_0008792 showed no influence on cell cycle of osteosarcoma cells.
CONCLUSION
Osteosarcoma is suppressed by hsa_circ_0008792/hsa-miR-711/ZFP1 axis.
目的
环状RNA是一种新发现的非编码RNA。它在调节基因表达中发挥重要作用，并可能参与肿瘤进展。本研究旨在探讨hsa_circ_0008792在骨肉瘤调控中的功能。
方法
我们通过生物信息学分析GSE96962数据集鉴定出一种环状RNA，即hsa_circ_0008792。采用伤口愈合试验和Transwell Matrigel试验评估迁移和侵袭能力。使用流式细胞术测量细胞周期中G0/G1、S和G2/M期的比例以及细胞凋亡情况。
结果
hsa_circ_0008792在骨肉瘤细胞中低表达，上调hsa_circ_0008792可抑制骨肉瘤细胞迁移和侵袭并促进细胞凋亡。这种调控由hsa-miR-711/ZFP1介导。hsa_circ_0008792的表达水平对骨肉瘤细胞的细胞周期无影响。
结论
hsa_circ_0008792/hsa-miR-711/ZFP1轴可抑制骨肉瘤。
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