Department of Gastroenterology, The Chinese People's Liberation Army Navy 971 Hospital, Qingdao, China.
J Clin Lab Anal. 2020 Jun;34(6):e23235. doi: 10.1002/jcla.23235. Epub 2020 Mar 25.
Gastric carcinoma (GC) is a familiar carcinoma and serious threat to human health. We investigated the efficacy and mechanism of circular RNA hsa_circ_0001649 on the growth, migration, and invasion of GC cells.
microRNA (miR)-20a and hsa_circ_0001649 expression was investigated by RT-qPCR and was changed by cell transfection. CCK-8, flow cytometry, and BrdU assays were, respectively, used to investigate the efficacies of hsa_circ_0001649 and miR-20a on cell viability, apoptosis, and proliferation. Transwell assay was used to investigate the efficacies of hsa_circ_0001649 and miR-20a on cell migration and invasion. Moreover, the levels of cyclin D1, Bax, cleaved caspase-3, and signal pathway-related proteins were investigated by Western blot.
Hsa_circ_0001649 was downregulated in GC cells and tissues. Upregulation of hsa_circ_0001649 restrained viability, proliferation, migration, and invasion, while promoted apoptosis. Furthermore, miR-20a was negatively regulated by hsa_circ_0001649 and miR-20a overexpression reversed the efficacy of hsa_circ_0001649 upregulation. Finally, upregulation of hsa_circ_0001649 restrained ERK and Wnt/β-catenin pathways while miR-20a overexpression reversed these progresses.
Upregulation of hsa_circ_0001649 restrained GC cell growth and metastasis by downregulating miR-20a and thereby inactivated ERK and Wnt/β-catenin pathways.
胃癌（GC）是一种常见的癌症，严重威胁人类健康。我们研究了环状 RNA hsa_circ_0001649 对 GC 细胞生长、迁移和侵袭的疗效和机制。
通过 RT-qPCR 检测 microRNA (miR)-20a 和 hsa_circ_0001649 的表达，并通过细胞转染改变其表达。CCK-8、流式细胞术和 BrdU 检测分别用于研究 hsa_circ_0001649 和 miR-20a 对细胞活力、凋亡和增殖的作用。Transwell 检测用于研究 hsa_circ_0001649 和 miR-20a 对细胞迁移和侵袭的作用。此外，通过 Western blot 检测细胞周期蛋白 D1、Bax、caspase-3 切割和信号通路相关蛋白的水平。
hsa_circ_0001649 在 GC 细胞和组织中下调。hsa_circ_0001649 的上调抑制了细胞活力、增殖、迁移和侵袭，同时促进了细胞凋亡。此外，hsa_circ_0001649 负调控 miR-20a，miR-20a 的过表达逆转了 hsa_circ_0001649 上调的作用。最后，hsa_circ_0001649 的上调抑制了 ERK 和 Wnt/β-catenin 通路，而 miR-20a 的过表达逆转了这些进展。
hsa_circ_0001649 的上调通过下调 miR-20a 抑制 GC 细胞生长和转移，从而抑制了 ERK 和 Wnt/β-catenin 通路。
