Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, Republic of Korea.
Department of Chemistry and Cosmetics, Jeju National University, Jeju 63243, Republic of Korea;, Email:
Pharmazie. 2020 Mar 20;75(2):107-111. doi: 10.1691/ph.2020.9735.
: Melanin in the skin is the defense against the harmful UV radiation, which is considered as one of the major risk factors for skin cancer. The compound 7,8-dimethoxycoumarin (DMC, CHO₄), a natural coumarin molecule present in several medicinal plants, possesses antioxidant and anti-inflammatory activities. However, the mechanism underlying its effects on melanogenesis in melanocytes is unclear. Therefore, we investigated the effect of DMC on melanogenesis activation in B16F10 melanoma cells. : We examined the cytotoxic range of DMC on B16F10 melanoma cells and increased effects of melanogenesis, and intracellular tyrosinase activity. In addition, regulation mechanisms were assessed by Western blot analysis. : The results showed that DMC significantly increased melanin content and tyrosinase activity in the cells without being cytotoxic. Furthermore, DMC stimulated the expression of tyrosinase, TRP-1, TRP-2, and MITF thereby activating melanin production and Akt phosphorylation was increased in the Akt signaling pathway. on the contrary, interfering with the phosphorylation of ERK in the MAPKs pathway. : These results suggest that DMC may serve as a candidate for potential melanin-producing activator and anti-gray hair applications.
皮肤中的黑色素是抵御有害紫外线辐射的防御机制，而紫外线辐射被认为是皮肤癌的主要危险因素之一。7,8-二甲氧基香豆素（DMC，CHO₄）是一种存在于多种药用植物中的天然香豆素分子，具有抗氧化和抗炎作用。然而，其对黑素细胞中黑色素生成的影响的机制尚不清楚。因此，我们研究了 DMC 对 B16F10 黑素瘤细胞中黑色素生成激活的影响。
我们研究了 DMC 对 B16F10 黑素瘤细胞的细胞毒性范围以及对黑色素生成和细胞内酪氨酸酶活性的增强作用。此外，通过 Western blot 分析评估了调节机制。
结果表明，DMC 在没有细胞毒性的情况下显著增加了细胞中的黑色素含量和酪氨酸酶活性。此外，DMC 刺激了酪氨酸酶、TRP-1、TRP-2 和 MITF 的表达，从而激活了黑色素的生成，并且 Akt 信号通路中的 Akt 磷酸化增加，而 MAPKs 通路中的 ERK 磷酸化受到干扰。
这些结果表明，DMC 可能是一种潜在的黑色素生成激活剂和抗白发应用的候选药物。
