Sui Juanjuan, Qu Changqing, Yang Jingxia, Zhang Wenna, Ji Yuntao
1Engineering Technology Research Center of Anti-aging Chinese Herbal Medicine, Fuyang Normal University, Fuyang, 236037 Anhui China.
2Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, China Agricultural University, 2 Yuanmingyuan West Road, Haidian District, Beijing, 100193 China.
Acta Physiol Plant. 2019;41(7):126. doi: 10.1007/s11738-019-2915-9. Epub 2019 Jun 17.
is a deciduous tree native to eastern and southeastern Asia that has important culinary and cultural values. To expand current knowledge of the transcriptome and functional genomics in this species, a de novo transcriptome sequence analysis of young and mature leaf tissues of was performed using the Illumina platform. Over 8.1 Gb of data were generated, assembled into 64,541 unigenes, and annotated with known biological functions. Proteins involved in primary metabolite biosynthesis were identified based on similarities to known proteins, including some related to biosynthesis of carbohydrates, amino acids, lipids, and energy. Analysis of unigenes differentially expressed between young and mature leaves (transcriptomic libraries 'YL' and 'ML', respectively) showed that the KEGG pathways of phenylpropanoid, naringenin, lignin, cutin, suberin, and wax biosynthesis were significantly enriched in mature leaves. These results not only expand knowledge of transcriptome characteristics for this valuable species, but also provide a useful transcriptomic dataset to accelerate the researches on its metabolic mechanisms and functional genomics. This study can also further the understanding of unique aromatic metabolism and Chinese medicinal properties of
是一种原产于东亚和东南亚的落叶树，具有重要的烹饪和文化价值。为了扩展对该物种转录组和功能基因组学的现有认识，使用Illumina平台对其幼叶和成熟叶组织进行了从头转录组序列分析。生成了超过8.1Gb的数据，组装成64,541个单基因，并标注了已知的生物学功能。基于与已知蛋白质的相似性鉴定了参与初级代谢物生物合成的蛋白质，包括一些与碳水化合物、氨基酸、脂质和能量生物合成相关的蛋白质。对幼叶和成熟叶（分别为转录组文库“YL”和“ML”）之间差异表达的单基因分析表明，成熟叶中苯丙烷类、柚皮素、木质素、角质、木栓质和蜡生物合成的KEGG途径显著富集。这些结果不仅扩展了对这种有价值物种转录组特征的认识，还提供了一个有用的转录组数据集，以加速对其代谢机制和功能基因组学的研究。这项研究还可以进一步加深对独特芳香代谢和中药特性的理解。
