一种从细胞培养物中制备用于电子显微镜检查的病毒分离物的简单、快速方法。
A simple, rapid method for preparation of virus isolates from cell culture for electron microscopy.
作者信息
Skilling D E, Barlough J E, Berry E S, Smith A W
机构信息
Calicivirus Research Laboratory, College of Veterinary Medicine, Oregon State University, 97331-4802 Corvallis, Oregon USA.
出版信息
J Tissue Cult Methods. 1986;9(4):217-220. doi: 10.1007/BF01665758.
Abstract
A simple procedure for the rapid preparation of virus isolates from cell culture for negative-contrast electron microscopy was devised. Using only conventional centrifugation steps (i.e. without ultracentrifugation), the procedure produced consistent, fine-quality preparations of a variety of virus types differing in size/shape and buoyant density.
摘要
设计了一种从细胞培养物中快速制备病毒分离物用于负染色电子显微镜观察的简单方法。该方法仅使用常规离心步骤（即不进行超速离心），就能制备出各种大小/形状和浮力密度不同的病毒类型的高质量、一致性好的样本。
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