通过聚丙烯酰胺水凝胶电子显微镜检测病毒颗粒。
Detection of virus particles by electron microscopy with polyacrylamide hydrogel.
作者信息
Whitby H J, Rodgers F G
出版信息
J Clin Pathol. 1980 May;33(5):484-7. doi: 10.1136/jcp.33.5.484.
Abstract
The use of lyphogel to concentrate the number of virus particles in specimens for electron microscopic examination was studied in parallel with ultracentrifugation. One hundred faecal and urine samples were compared. Both methods had a similar sensitivity. Lyphogel was economical, simple, and rapid in use; in contrast to ultracentrifugation, it required relatively little material. The procedure could be done within a safety cabinet, and virus particles were morphologically undamaged by the process.
摘要
研究了使用冻干凝胶浓缩标本中的病毒颗粒数量以进行电子显微镜检查，并与超速离心法进行了对比。比较了100份粪便和尿液样本。两种方法的灵敏度相似。冻干凝胶经济、操作简单且快速；与超速离心法不同，它所需的材料相对较少。该操作可在安全柜内完成，且病毒颗粒在形态上未因该过程而受损。
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