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利用 DNA 标记物对市售人参产品中人参和西洋参的高通量鉴定的实际应用。

Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products.

机构信息

Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agriculture and Life Sciences, Seoul National University, Seoul, Korea.

National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, Korea.

出版信息

J Ginseng Res. 2014 Apr;38(2):123-9. doi: 10.1016/j.jgr.2013.11.017. Epub 2013 Dec 17.

DOI:10.1016/j.jgr.2013.11.017
PMID:24748836
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3986582/
Abstract

Korean ginseng (Panax ginseng) and American ginseng (Panax quinquefolius) are widely used medicinal plants with similar morphology but different medicinal efficacy. Roots, flowers, and processed products of Korean and American ginseng can be difficult to differentiate from each other, leading to illegal trade in which one species is sold as the other. This study was carried out to develop convenient and reliable chloroplast genome-derived DNA markers for authentication of Korean and American ginseng in commercial processed products. One codominant marker could reproducibly identify both species and intentional mixtures of the two species. We further developed a set of species-unique dominant DNA markers. Each species-specific dominant marker could detect 1% cross contamination with other species by low resolution agarose gel electrophoresis or quantitative polymerase chain reaction. Both markers were successfully applied to evaluate the original species from various processed ginseng products purchased from markets in Korea and China. We believe that high-throughput application of this marker system will eradicate illegal trade and promote confident marketing for both species to increase the value of Korean as well as American ginseng in Korea and worldwide.

摘要

高丽参（Panax ginseng）和西洋参（Panax quinquefolius）是两种广泛应用的药用植物，它们在形态上相似，但药用功效不同。高丽参和西洋参的根、花和加工品很难区分，导致一种植物被当作另一种植物进行非法交易。本研究旨在开发方便可靠的叶绿体基因组衍生 DNA 标记物，用于鉴定商业加工产品中的高丽参和西洋参。一种共显性标记物可以重复识别两种物种及其有意混合物。我们进一步开发了一组物种特异性的显性 DNA 标记物。每个种特异性的显性标记物可以通过低分辨率琼脂糖凝胶电泳或定量聚合酶链反应检测到 1%的与其他物种的交叉污染。这两个标记物都成功地应用于评估从韩国和中国市场购买的各种加工人参产品的原始物种。我们相信，这种标记系统的高通量应用将消除非法贸易，并为两种物种的自信营销提供支持，从而提高高丽参和西洋参在韩国和全球的价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/38e92ce32050/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/43909c21e313/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/7ce790394241/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/009856bf4db6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/3bc48f6cf060/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/00f9ba4732db/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/25775d809806/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/38e92ce32050/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/43909c21e313/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/7ce790394241/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/009856bf4db6/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/3bc48f6cf060/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/00f9ba4732db/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/25775d809806/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c182/3986582/38e92ce32050/gr7.jpg

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