Khaled A G A, Motawea M H, Said A A
Department of Genetics, Faculty of Agriculture, Sohag University, 82786 Sohag, Egypt.
Department of Agronomy, Faculty of Agriculture, Sohag University, Sohag 82786, Egypt.
J Genet Eng Biotechnol. 2015 Dec;13(2):243-252. doi: 10.1016/j.jgeb.2015.05.001. Epub 2015 May 29.
Genetic variability and identification of some molecular markers were studied in twenty promising lines of wheat using agronomic traits, ISSR (inter simple sequences repeats) and RAPD (random amplified polymorphic DNA) markers. Significant variation was evidenced in all agronomic traits. The lines proved to be superior to the check cultivar Sahel1 in yield and its component traits. Lines L2, L7 and L8 were the best in most yield component traits in both seasons. Moreover, Lines L2, L4, L5, L7 and L8 showed drought tolerance by which they displayed high performance in agronomic traits as well as a low drought susceptibility index. The percentage of polymorphism was 39.3% and 53.2% for ISSRs and RAPDs, respectively. UBC-881 belonged to penta-nucleotide repeat sequences (GGGTG) that produced the highest level of polymorphism, while UBC-846 belonged to di-nucleotide repeat sequences (CA) that produced the lowest level of polymorphism. Genetic similarities among wheat lines based on ISSR and RAPD markers ranged from 0.81 to 1.00 and from 0.86 to 0.98, respectively. There was a low average of PIC (polymorphism information content) values which were 0.10 (ISSR) and 0.15 (RAPD). The RAPD technique exhibited a higher marker index (MI = 0.69) compared to ISSR (MI = 0.43). There was insignificant correlation between ISSR and RAPD data (0.168, > 0.05). There were two markers (UBC-881 and OPF-10), on each of which two traits regressed significantly. The associated markers each explained a maximum regression of 18.92-34.95% of the total available variation for individual associated traits.
利用农艺性状、ISSR（简单序列重复区间）和RAPD（随机扩增多态性DNA）标记，对20个优良小麦品系的遗传变异性和一些分子标记进行了研究。所有农艺性状均表现出显著变异。这些品系在产量及其构成性状方面优于对照品种萨赫勒1号。品系L2、L7和L8在两个季节的大多数产量构成性状方面表现最佳。此外，品系L2、L4、L5、L7和L8表现出耐旱性，它们在农艺性状方面表现良好，且干旱敏感指数较低。ISSR和RAPD的多态性百分比分别为39.3%和53.2%。UBC - 881属于五核苷酸重复序列（GGGTG），产生的多态性水平最高，而UBC - 846属于二核苷酸重复序列（CA），产生的多态性水平最低。基于ISSR和RAPD标记的小麦品系间遗传相似性分别为0.81至1.00和0.86至0.98。PIC（多态性信息含量）值的平均值较低，分别为0.10（ISSR）和0.15（RAPD）。与ISSR（MI = 0.43）相比，RAPD技术表现出更高的标记指数（MI = 0.69）。ISSR和RAPD数据之间的相关性不显著（0.168，> 0.05）。有两个标记（UBC - 881和OPF - 10），每个标记上有两个性状显著回归。每个相关标记对单个相关性状的总可用变异的最大回归解释率为18.92 - 34.95%。
