异欧前胡素通过MAPK/ERK1/2信号通路诱导鼻咽癌细胞凋亡。
Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway.
作者信息
Liu Jie, He Lan, Hu Jing, Li Kairui, Zhou Fangliang, Hu Mei, Luo Jingjing, Song Lan, He Yingchun
机构信息
Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China.
Medicine School, Hunan University of Chinese, Changsha, Hunan 410208, China.
出版信息
Evid Based Complement Alternat Med. 2020 Mar 9;2020:2138186. doi: 10.1155/2020/2138186. eCollection 2020.
OBJECTIVE
To investigate the effect of isoimperatorin on nasopharyngeal carcinoma CNE2 cell apoptosis and the role of the MAPK/ERK1/2 signaling pathway in inducing apoptosis.
METHODS
Real-time cellular analysis technology (RTCA) and MTT were used to detect cell proliferation; Annexin V-FITC/PI dual-fluorescence flow cytometry analysis, Hoechst 33342 staining, and mitochondrial membrane potential detection kit were used to detect cell apoptosis; western blot was used to detect protein expression.
RESULTS
Different concentrations of isoimperatorin (10 M, 20 M, 20 M, 20 M, 20 M, 20 M, 20 M, 20 .
CONCLUSION
Isoimperatorin can induce nasopharyngeal carcinoma CNE2 cell apoptosis through the MAPK/ERK1/2 signaling pathway.
目的
探讨异欧前胡素对鼻咽癌CNE2细胞凋亡的影响以及丝裂原活化蛋白激酶/细胞外信号调节激酶1/2（MAPK/ERK1/2）信号通路在诱导凋亡中的作用。
方法
采用实时细胞分析技术（RTCA）和MTT法检测细胞增殖；用膜联蛋白V-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）双荧光流式细胞术分析、Hoechst 33342染色及线粒体膜电位检测试剂盒检测细胞凋亡；用蛋白质免疫印迹法检测蛋白表达。
结果
不同浓度的异欧前胡素（10μM、20μM、20μM、20μM、20μM、20μM、20μM、20…
结论
异欧前胡素可通过MAPK/ERK1/2信号通路诱导鼻咽癌CNE2细胞凋亡。
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