Pol Françoise, Dorenlor Virginie, Eono Florent, Eudier Solveig, Eveno Eric, Liégard-Vanhecke Dorine, Rose Nicolas, Fablet Christelle
Agence Nationale de Sécurité Sanitaire (Anses), B.P. 53, F-22440 Ploufragan, France; Université Bretagne-Loire, France.
Agence Nationale de Sécurité Sanitaire (Anses), B.P. 53, F-22440 Ploufragan, France; Université Bretagne-Loire, France.
Prev Vet Med. 2017 Nov 1;147:58-65. doi: 10.1016/j.prevetmed.2017.08.011. Epub 2017 Sep 6.
The aims of this study were to assess the feasibility of individual and pen-based oral fluid sampling (OFS) in 35 pig herds with group-housed sows, compare these methods to blood sampling, and assess the factors influencing the success of sampling. Individual samples were collected from at least 30 sows per herd. Pen-based OFS was performed using devices placed in at least three pens for 45min. Information related to the farm, the sows, and their living conditions were collected. Factors significantly associated with the duration of sampling and the chewing behaviour of sows were identified by logistic regression. Individual OFS took 2min 42s on average; the type of floor, swab size, and operator were associated with a sampling time >2min. Pen-based OFS was obtained from 112 devices (62.2%). The type of floor, parity, pen-level activity, and type of feeding were associated with chewing behaviour. Pen activity was associated with the latency to interact with the device. The type of floor, gestation stage, parity, group size, and latency to interact with the device were associated with a chewing time >10min. After 15, 30 and 45min of pen-based OFS, 48%, 60% and 65% of the sows were lying down, respectively. The time spent after the beginning of sampling, genetic type, and time elapsed since the last meal were associated with 50% of the sows lying down at one time point. The mean time to blood sample the sows was 1min 16s and 2min 52s if the number of operators required was considered in the sampling time estimation. The genetic type, parity, and type of floor were significantly associated with a sampling time higher than 1min 30s. This study shows that individual OFS is easy to perform in group-housed sows by a single operator, even though straw-bedded animals take longer to sample than animals housed on slatted floors, and suggests some guidelines to optimise pen-based OFS success.
本研究的目的是评估在35个饲养群养母猪的猪场中进行个体和基于栏舍的口腔液体采样（OFS）的可行性，将这些方法与血液采样进行比较，并评估影响采样成功的因素。每个猪群至少从30头母猪中采集个体样本。基于栏舍的OFS使用放置在至少三个栏舍中的设备进行45分钟。收集了与猪场、母猪及其生活条件相关的信息。通过逻辑回归确定了与采样持续时间和母猪咀嚼行为显著相关的因素。个体OFS平均耗时2分42秒；地面类型、拭子大小和操作人员与采样时间>2分钟相关。从112个设备（62.2%）获得了基于栏舍的OFS。地面类型、胎次、栏舍水平活动和饲养类型与咀嚼行为相关。栏舍活动与与设备互动的潜伏期相关。地面类型、妊娠阶段、胎次、群体大小和与设备互动的潜伏期与咀嚼时间>10分钟相关。在基于栏舍的OFS进行15、30和45分钟后，分别有48%、60%和65%的母猪躺下。采样开始后的时间、遗传类型和自上次进食以来经过的时间与在一个时间点有50%的母猪躺下相关。如果在采样时间估计中考虑所需操作人员数量，给母猪采集血样的平均时间为1分16秒和2分52秒。遗传类型、胎次和地面类型与高于1分30秒的采样时间显著相关。本研究表明，个体OFS由单个操作人员在群养母猪中易于实施，尽管垫草饲养的动物比板条地面饲养的动物采样时间更长，并提出了一些优化基于栏舍的OFS成功的指导原则。
