Selmi Marwa, Belmabrouk Hafedh
Department of Radiological Sciences and Medical Imaging, College of Applied Medical Sciences, Majmaah University, Majmaah 11952, Saudi Arabia.
Laboratory of Electronics and Microelectronics, Faculty of Science of Monastir, University of Monastir, Environment Boulevard, Monastir 5019, Tunisia.
Micromachines (Basel). 2020 Mar 25;11(4):342. doi: 10.3390/mi11040342.
A heterogeneous immunoassay is an efficient biomedical test. It aims to detect the presence of an analyte or to measure its concentration. It has many applications, such as manipulating particles and separating cancer cells from blood. The enhanced performance of immunosensors comes down to capturing more antigens with greater efficiency by antibodies in a short time. In this work, we report an efficient investigation of the effects of alternating current (AC) electrokinetic forces such as AC electroosmosis (ACEO), which arise when the fluid absorbs energy from an applied electric field, on the kinetics of the antigen-antibody binding in a flow system. The force can produce swirling structures in the fluid and, thus, improve the transport of the analyte toward the reaction surface of the immunosensor device. A numerical simulation is adequate for this purpose and may provide valuable information. The convection-diffusion phenomenon is coupled with the first-order Langmuir model. The governing equations are solved using the finite element method (FEM). The impact of AC electroosmosis on the binding reaction kinetics, the fluid flow stream modification, the analyte concentration diffusion, and the detection time of the biosensor under AC electroosmosis are analyzed.
非均相免疫测定是一种高效的生物医学检测方法。它旨在检测分析物的存在或测量其浓度。它有许多应用，例如操控颗粒以及从血液中分离癌细胞。免疫传感器性能的提升归结于抗体在短时间内更高效地捕获更多抗原。在这项工作中，我们报告了一项关于交流（AC）电动作用力（如交流电渗（ACEO））影响的有效研究，当流体从施加的电场吸收能量时会产生这种交流电渗，其对流动系统中抗原 - 抗体结合动力学的影响。这种力可在流体中产生涡旋结构，从而改善分析物向免疫传感器装置反应表面的传输。数值模拟适用于此目的，并且可能提供有价值的信息。对流 - 扩散现象与一级朗缪尔模型相耦合。使用有限元方法（FEM）求解控制方程。分析了交流电渗对结合反应动力学、流体流动流型改变、分析物浓度扩散以及交流电渗下生物传感器检测时间的影响。
