Witkowski Peter T, Heinemann Patrick, Klempa Boris, Krüger Detlev H
Institut für Medizinische Virologie, Helmut-Ruska-Haus, Charité-Universitätsmedizin Berlin, Helmut-Ruska-Haus Charitéplatz 1, D-10117 Berlin, Germany.
Biospektrum (Heidelb). 2015;21(5):503-506. doi: 10.1007/s12268-015-0609-4. Epub 2015 Aug 27.
In addition to classical virus isolation in cell culture, the molecular detection of new virus variants by PCR techniques allows broader epidemiological insights into the world of viral pathogens. For the detection of hantaviruses-zoonotic viruses leading to fever and organ failure in humans-we developed a genus-wide nested RT-PCR format, which enables the discovery of new members within this virus genus. The methodological approach allowed the demonstration of first hantaviruses from Africa and revealed new hantavirus reservoir hosts, as shrews, moles, and bats.
除了在细胞培养中进行经典的病毒分离外，通过PCR技术对新病毒变种进行分子检测，能够更广泛地洞察病毒病原体的流行病学情况。为了检测汉坦病毒（一种导致人类发热和器官衰竭的人畜共患病毒），我们开发了一种全属嵌套式逆转录PCR方法，该方法能够发现该病毒属内的新成员。这种方法首次证实了非洲存在汉坦病毒，并揭示了新的汉坦病毒宿主，如鼩鼱、鼹鼠和蝙蝠。
