Trolle Jensen Theis Zetner, Mackie Meaghan, Taurozzi Alberto John, Lanigan Liam Thomas, Gundelach Carsten, Olsen Jesper, Sørensen Søren Anker, Collins Matthew James, Sørensen Mikkel, Schroeder Hannes
Section for Evolutionary Genomics, The Globe Institute, Faculty of Health, University of Copenhagen, Øster Voldgade 5-7, 1350 Copenhagen, Denmark.
BioArCh, Department of Archaeology, Environment Building, Wentworth Way, University of York, York YO10 5NG, UK.
R Soc Open Sci. 2020 Jan 22;7(1):191172. doi: 10.1098/rsos.191172. eCollection 2020 Jan.
We present the analysis of an osseous finger ring from a predominantly early Neolithic context in Denmark. To characterize the artefact and identify the raw material used for its manufacture, we performed micro-computed tomography scanning, zooarchaeology by mass spectrometry (ZooMS) peptide mass fingerprinting, as well as protein sequencing by liquid chromatography tandem mass spectrometry (LC-MS/MS). We conclude that the ring was made from long bone or antler due to the presence of osteons (Haversian canals). Subsequent ZooMS analysis of collagen I and II indicated that it was made from or material. We then used LC-MS/MS analysis to refine our species identification, confirming that the ring was made from , and to examine the rest of the proteome. This study demonstrates the potential of ancient proteomics for species identification of prehistoric artefacts made from osseous material.
我们展示了对一枚来自丹麦新石器时代早期为主的骨质指环的分析。为了描述该文物的特征并确定其制造所用的原材料，我们进行了微计算机断层扫描、质谱动物考古学（ZooMS）肽质量指纹分析以及液相色谱串联质谱（LC-MS/MS）蛋白质测序。我们得出结论，由于存在骨单位（哈弗斯管），该指环是由长骨或鹿角制成的。随后对I型和II型胶原蛋白的ZooMS分析表明，它是由 或 材料制成的。然后我们使用LC-MS/MS分析来完善我们的物种鉴定，确认该指环是由 制成的，并检查蛋白质组的其余部分。这项研究证明了古代蛋白质组学在鉴定由骨质材料制成的史前文物物种方面的潜力。
