Xu Dejun, Wu Lin, Yang Li, Liu Dingbang, Chen Huali, Geng Guoxia, Li Qingwang
College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, China.
College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling, China.
Anim Sci J. 2020 Jan-Dec;91(1):e13328. doi: 10.1111/asj.13328.
This study was aimed to investigate whether and how Rutin protects boar sperm against cryoinjury during cryopreservation. Five concentrations of Rutin with 0.2, 0.4, 0.6, 1.0, and 2.0 mM were added to the freezing extender of boar sperm, respectively, and the effects on quality and function of boar sperm after freezing-thawing were assessed. The results showed that the sperm motility, mitochondrial activity, plasma membrane integrity, and acrosomal integrity were significantly improved in 0.4 mM and 0.6 mM Rutin groups (p < .05). Compared with ganoderma lucidum polysaccharide (GLP) or Tanshinone IIA, Rutin exhibited higher rates of mitochondrial activity and acrosome integrity (p < .05). Mechanistically, the addition of Rutin at the concentration of 0.6, 0.8, and 1.0 mM significantly attenuated ROS accumulation and MDA production by improving antioxidant enzymatic activity, including SOD, CAT, and GSH-Px (p < .05). Functionally, a higher penetration rate and the increased total efficiency of fertilization were observed in the 0.4, 0.6, and 1.0 mM Rutin groups than in the control group (p < .05). Moreover, the addition of Rutin (0.6 mM) significantly induced an increase in both the cleavage and blastocyst rates (p < .05). In summary, supplementation with Rutin in cryopreservation medium protects boar sperm against ROS attack by enhancing the antioxidative defense.
本研究旨在探讨芦丁在猪精子冷冻保存过程中是否以及如何保护其免受冷冻损伤。分别向猪精子冷冻稀释液中添加浓度为0.2、0.4、0.6、1.0和2.0 mM的五种芦丁溶液，并评估其对冻融后猪精子质量和功能的影响。结果表明，0.4 mM和0.6 mM芦丁组的精子活力、线粒体活性、质膜完整性和顶体完整性均显著提高（p < 0.05）。与灵芝多糖（GLP）或丹参酮IIA相比，芦丁的线粒体活性和顶体完整性率更高（p < 0.05）。机制上，添加浓度为0.6、0.8和1.0 mM的芦丁通过提高抗氧化酶活性（包括超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶）显著减弱了活性氧的积累和丙二醛的产生（p < 0.05）。功能上，0.4、0.6和1.0 mM芦丁组的穿透率和总受精效率均高于对照组（p < 0.05）。此外，添加芦丁（0.6 mM）显著提高了卵裂率和囊胚率（p < 0.05）。总之，在冷冻保存培养基中添加芦丁可通过增强抗氧化防御来保护猪精子免受活性氧攻击。
