Barker W C, Hunt L T, George D G
Protein Identification Resource, National Biomedical Research Foundation, Washington, D.C. 20007.
Protein Seq Data Anal. 1988;1(5):363-73.
Amino acid sequences are often inferred without information about the structure or function of the protein. Just as unknown proteins may be identified through comparison with other known sequences, so can structural and functional domains be inferred through analogy with other known domain sequences. A database of domain sequences, along with their known properties, would be very helpful in the characterization of a protein sequence in terms of its possible conformation and function. Here we describe the type of information to be included in such a database and discuss the techniques that may be used to determine the characteristic features of domains. Recently identified domains in thyroid peroxidase, chondroitin-sulfate proteoglycan core proteins, an epidermal growth factor precursor homolog, and a bacterial beta-amylase are presented as examples.
氨基酸序列通常在没有蛋白质结构或功能信息的情况下推断得出。正如未知蛋白质可通过与其他已知序列比较来鉴定一样，结构域和功能域也可通过与其他已知结构域序列类比来推断。一个包含结构域序列及其已知特性的数据库，对于根据蛋白质序列可能的构象和功能对其进行表征将非常有帮助。在此，我们描述此类数据库应包含的信息类型，并讨论可用于确定结构域特征的技术。最近在甲状腺过氧化物酶、硫酸软骨素蛋白聚糖核心蛋白、表皮生长因子前体同源物和细菌β-淀粉酶中鉴定出的结构域作为示例给出。
