Forcados Gilead Ebiegberi, Sallau Abdullahi Balarabe, Muhammad Aliyu, Erukainure Ochuko Lucky, James Dorcas Bolanle
Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Nigeria.
Nutrition and Toxicology Division, Federal Institute of Industrial Research, Oshodi, Lagos, Nigeria.
Nutr Cancer. 2021;73(1):98-112. doi: 10.1080/01635581.2020.1743866. Epub 2020 Mar 28.
leaves are used traditionally in West Africa for the treatment of swellings and cancer. We investigated if leaves extract could ameliorate 7, 12-dimethylbenz[a]anthracene (DMBA)-induced mammary damage. Female Wistar rats aged 52 ± 2 day were administered 80 mg/kg DMBA. After monitoring for 150 day, rats were administered 0, 50, 100, 200 mg/kg and 20 mg/kg Tamoxifen for 14 day. Serum estrogen receptor-α, IL-1β and TNF -α levels were determined using ELISA kits. Oxidative stress markers in mammary tissue homogenates were determined using standard spectrophotometric methods. Histopathological examination was done using hematoxylin and eosin staining and cyclooxygenase-2 (COX-2) expression using immunohistochemistry. Liquid chromatography-mass spectrometry was used to determine components present in the extract. Although tumors were not observed, significantly ( < 0.05) lower estrogen receptor-α, malondialdehyde, IL-1β and TNF -α levels, significantly ( < 0.05) higher glutathione and catalase activity, attenuation of malignant epithelial hyperplasia and mild COX-2 expression were observed in rats administered when compared to DMBA-induced untreated control. Liquid chromatography-mass spectrometry analysis of the extract revealed the presence of 4,5-dihydroxy-7-methoxy-6-methylflavone and vanillylamine, which are compounds with reported antioxidant and anti-inflammatory effects. Collectively, treatment with ameliorated some derangement observed in DMBA-induced rats.
在西非，树叶传统上被用于治疗肿胀和癌症。我们研究了树叶提取物是否能改善7,12-二甲基苯并[a]蒽（DMBA）诱导的乳腺损伤。对52±2日龄的雌性Wistar大鼠给予80mg/kg DMBA。监测150天后，给大鼠分别灌胃0、50、100、200mg/kg以及20mg/kg他莫昔芬，持续14天。使用ELISA试剂盒测定血清雌激素受体-α、IL-1β和TNF-α水平。采用标准分光光度法测定乳腺组织匀浆中的氧化应激标志物。使用苏木精和伊红染色进行组织病理学检查，并通过免疫组织化学检测环氧合酶-2（COX-2）的表达。采用液相色谱-质谱联用技术测定提取物中的成分。虽然未观察到肿瘤，但与DMBA诱导的未处理对照组相比，给予提取物的大鼠雌激素受体-α、丙二醛、IL-1β和TNF-α水平显著降低（<0.05），谷胱甘肽和过氧化氢酶活性显著升高（<0.05），恶性上皮增生减轻，COX-2表达轻度降低。提取物的液相色谱-质谱联用分析显示存在4,5-二羟基-7-甲氧基-6-甲基黄酮和香草胺，这些化合物具有抗氧化和抗炎作用。总体而言，提取物治疗改善了DMBA诱导大鼠中观察到的一些紊乱情况。
