Laboratory of Water Pollution Remediation, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Laboratory of Water Pollution Remediation, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Sci Total Environ. 2020 Jun 25;723:138094. doi: 10.1016/j.scitotenv.2020.138094. Epub 2020 Mar 21.
The preservation of anaerobic ammonia oxidation (anammox) consortia is crucial for the rapid start-up and the process stability of the anammox based bioreactor. This work proposed and evaluated the feasibility of an anammox consortia preservation strategy, in which the anammox sludge was transformed into intermediate anoxic sulfide oxidation (ASO) functional microorganisms. Initially, the ASO process was successfully started up by inoculating anammox sludge and the overall sulfide and nitrate removal rates stabilized at 57.5 ± 0.22 and 10.0 ± 0.18 kg m day, respectively. Then, the bioreactor function was reversely transformed into anammox, whose nitrogen removal rate reached 1.68 kg m day. Granule characteristics analysis revealed that both biomass and extracellular polymeric substance content returned to their original states after the reverse start-up. Although the population of Candidatus_Kuenenia was greatly declined during ASO process, its richness was successfully recovered after the reverse start-up of the anammox process. The inferred metagenomes analysis demonstrated that the shifts in functional microorganisms were related to variation in the main metabolic pathways. The specific activities of anammox and ASO both are regarded as key indicators for the successful start-up of bioreactor. This work revealed a novel technique for the preservation of anammox consortia and might be a potential strategy for overcoming the drawback of long start-up time.
厌氧氨氧化（Anammox）菌的保存对于基于 Anammox 的生物反应器的快速启动和过程稳定性至关重要。本研究提出并评估了一种 Anammox 菌保存策略的可行性，即将 Anammox 污泥转化为中间缺氧硫化物氧化（ASO）功能微生物。首先，通过接种 Anammox 污泥成功启动了 ASO 过程，整体硫化物和硝酸盐去除率分别稳定在 57.5 ± 0.22 和 10.0 ± 0.18 kg m-3 day-1。然后，生物反应器功能反向转化为 Anammox，其氮去除速率达到 1.68 kg m-3 day-1。颗粒特性分析表明，反向启动后生物量和胞外聚合物含量均恢复到原始状态。虽然在 ASO 过程中 Candidatus_Kuenenia 的种群数量大大减少，但在 Anammox 过程的反向启动后，其丰富度得到了成功恢复。推断的宏基因组分析表明，功能微生物的变化与主要代谢途径的变化有关。Anammox 和 ASO 的比活性都被认为是生物反应器成功启动的关键指标。本研究揭示了一种保存 Anammox 菌的新方法，可能是克服启动时间长的缺点的潜在策略。
