Ben-Ishay Z, Sharon S
Scand J Haematol. 1977 Mar;18(3):226-34. doi: 10.1111/j.1600-0609.1977.tb02334.x.
A single dose of hydroxyurea (HU) (400 mg/kg body weight) was injected i.p. into rats. Hydroxyurea, a potent DNA synthesis inhibitor, killed bone marrow cells in the S-phase of the cell cycle. 9-10 h after HU administration, the rat bone marrow contained large numbers of necrotic cells and appeared predominantly lymphoid. Within 24-48 h of HU injection, resumption of normal haematopoiesis was observed. Rat bone marrow, 9-10 h following HU administration, was implanted in peritoneal diffusion chambers (DC) in irradiated mice. The results obtained suggest that pretreatment of donor rats with HU increases the bone marrow content of progenitor cells. The stem cell-enriched marrow was conductive to enhanced growth of DC colonies in irradiated mice.
向大鼠腹腔注射单剂量羟基脲（HU）（400 mg/kg体重）。羟基脲是一种有效的DNA合成抑制剂，可杀死细胞周期S期的骨髓细胞。给予HU后9 - 10小时，大鼠骨髓含有大量坏死细胞，且主要呈现淋巴细胞样。在注射HU后的24 - 48小时内，观察到正常造血功能的恢复。在给予HU后9 - 10小时的大鼠骨髓被植入受辐照小鼠的腹膜扩散盒（DC）中。所得结果表明，用HU预处理供体大鼠可增加祖细胞的骨髓含量。富含干细胞的骨髓有助于受辐照小鼠中DC集落的生长增强。
