Ben-Ishay Z, Sharon S, Reichert F
Acta Haematol. 1978;59(2):88-98. doi: 10.1159/000207750.
Donor rats of the Hebrew University strain were administered a single intraperitoneal injection of hydroxyurea (400 mg/kg body weight). 1--3 h following the administration of the drug, a suspension of spleen cells, the majority of which consisted of lymphocytes, was prepared. Spleen cells were placed in diffusion chambers and these were implanted in the peritoneal cavity of preirradiated mice. 5--8 days following implantation, erythroid and granulocytic colonies developed in 30.3% of the diffusion chambers studied. However, in most chambers, macrophages were observed. In control experiments with implantation of spleen cells of normal rats, granulocytic colonies did not grow and in only 3.1% of the chambers erythroid colonies were noted. Macrophage colonies, however, developed in all 32 control cultures. Our previous studies showed that administration of a single dose of hydroxyurea strips the rat bone marrow of approximately 50% of replicating cells within 9--10 h. The results of the present study indicate that such a severe depletion of rat marrow cells results in early committment of spleen stem cells to various blood cell lines.
给希伯来大学品系的供体大鼠腹腔内单次注射羟基脲（400毫克/千克体重）。给药后1至3小时，制备了脾细胞悬液，其中大部分是淋巴细胞。将脾细胞置于扩散小室中，并将其植入预先照射过的小鼠腹腔内。植入后5至8天，在所研究的30.3%的扩散小室中出现了红系和粒系集落。然而，在大多数小室中观察到的是巨噬细胞。在正常大鼠脾细胞植入的对照实验中，粒系集落未生长，仅在3.1%的小室中发现了红系集落。然而，在所有32个对照培养物中都形成了巨噬细胞集落。我们之前的研究表明，单次给予羟基脲能在9至10小时内使大鼠骨髓中约50%的复制细胞减少。本研究结果表明，大鼠骨髓细胞如此严重的减少会导致脾干细胞早期向各种血细胞系定向分化。
