Cavalcanti Rayza Morganna Farias, Martinez Marcelo Luís Lombardi, Oliveira Wanderley Pereira, Guimarães Luís Henrique Souza
1Instituto de Química de Araraquara - UNESP, Avenida Professor Mário Degni s/nº, Quitandinha, Araraquara, SP 14800-900 Brazil.
2Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, USP, Av. do Café s/n, Ribeirão Preto, SP 14040-903 Brazil.
3 Biotech. 2020 Apr;10(4):177. doi: 10.1007/s13205-020-2164-z. Epub 2020 Mar 25.
The CAS21 tannase was spray dried with β-cyclodextrin, Capsul starch, soybean meal, lactose, and maltodextrin as adjuvants. The moisture content and water activity of the products ranged from 5.6 to 11.5% and from 0.249 to 0.448, respectively. The maximal tannase activity was achieved at 40-60 ºC and pH 5.0-6.0 for the powders containing β-cyclodextrin and Capsul starch, which was stable at 40 ºC and 40-60 ºC for 120 min, respectively. For all the dried products, tannase retained its activity of over 80% for 120 min at pH 5.0 and 6.0. Salts and solvents influenced the activity of the spray-dried tannase. The activity of the spray-dried tannase was maintained when preserved for 1 year at 4 ºC and 28 ºC. Spray-dried tannase reduced the content of tannins and polyphenolic compounds of leather effluent and sorghum flour and catalyzed the transesterification reaction. The spray drying process stabilized the tannase activity, highlighting the potential of dried products for biotechnological applications.
将CAS21单宁酶与β-环糊精、胶囊淀粉、豆粕、乳糖和麦芽糊精作为辅料进行喷雾干燥。产品的水分含量和水分活度分别为5.6%至11.5%和0.249至0.448。对于含有β-环糊精和胶囊淀粉的粉末，在40 - 60℃和pH 5.0 - 6.0时可达到最大单宁酶活性，分别在40℃和40 - 60℃下稳定120分钟。对于所有干燥产品，单宁酶在pH 5.0和6.0时120分钟内保持其80%以上的活性。盐和溶剂会影响喷雾干燥单宁酶的活性。喷雾干燥的单宁酶在4℃和28℃下保存1年时活性得以保持。喷雾干燥的单宁酶降低了皮革废水和高粱粉中单宁和多酚化合物的含量，并催化了酯交换反应。喷雾干燥过程稳定了单宁酶的活性，突出了干燥产品在生物技术应用中的潜力。
