Departamento de Engenharia de Bioprocessos e Biotecnologia, Universidade Federal do Paraná-UFPR, Curitiba, PR, 81531-980, Brazil.
Appl Biochem Biotechnol. 2020 Jan;190(1):113-128. doi: 10.1007/s12010-019-03075-1. Epub 2019 Jul 13.
Raw domestic wastewater was used as a culture medium for cellulase production in a bubble column reactor (6.2 U/mL, 64.6 U/L h) using the strain Trichoderma harzianum TRIC03-LPBII. Cellulases presented optimum pH and temperature between 4 and 5 and 50 and 70 °C, respectively. Enzymatic extract was concentrated through ultrafiltration and then a cellulolytic formulation was prepared with the addition of sorbitol (50% w/v) and benzoic acid (0.05% w/v). High cellulase stability of around 100% was reached after 30 days at 4 °C. The concentrated extract was also dried in a spray-dryer with the addition of maltodextrin at 20% (w/v), resulting in powder enzymatic formulation with 85% stability after 60 days. With these characteristics, the liquid and powder cellulase products have potential to be used in different industrial applications.
采用里氏木霉 TRIC03-LPBII 菌株，在鼓泡柱式反应器中利用生活污水作为培养基生产纤维素酶（6.2 U/mL，64.6 U/L h）。纤维素酶的最适 pH 和温度分别为 4-5 和 50-70°C。通过超滤浓缩酶提取液，然后添加山梨醇（50% w/v）和苯甲酸（0.05% w/v）制备纤维素酶制剂。浓缩提取液在 4°C 下放置 30 天后，纤维素酶稳定性仍高达 100%左右。浓缩提取液还在喷雾干燥器中添加麦芽糊精（20% w/v）进行干燥，得到的粉末酶制剂在 60 天后仍具有 85%的稳定性。具有这些特性的液体和粉末纤维素酶产品具有在不同工业应用中应用的潜力。
