Bioinformatics Research Center, North Carolina State University, Raleigh, NC, 27695, USA.
Biostatistics and Computational Biology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC, 27709, USA.
Small. 2020 May;16(21):e2000299. doi: 10.1002/smll.202000299. Epub 2020 Mar 29.
Silver nanoparticles (AgNPs) are widely incorporated into consumer and biomedical products for their antimicrobial and plasmonic properties with limited risk assessment of low-dose cumulative exposure in humans. To evaluate cellular responses to low-dose AgNP exposures across time, human liver cells (HepG2) are exposed to AgNPs with three different surface charges (1.2 µg mL ) and complete gene expression is monitored across a 24 h period. Time and AgNP surface chemistry mediate gene expression. In addition, since cells are fed, time has marked effects on gene expression that should be considered. Surface chemistry of AgNPs alters gene transcription in a time-dependent manner, with the most dramatic effects in cationic AgNPs. Universal to all surface coatings, AgNP-treated cells responded by inactivating proliferation and enabling cell cycle checkpoints. Further analysis of these universal features of AgNP cellular response, as well as more detailed analysis of specific AgNP treatments, time points, or specific genes, is facilitated with an accompanying application. Taken together, these results provide a foundation for understanding hepatic response to low-dose AgNPs for future risk assessment.
银纳米粒子（AgNPs）因其具有抗菌和等离子体特性而被广泛应用于消费和生物医学产品中，但对人类低剂量累积暴露的风险评估有限。为了评估细胞对低剂量 AgNP 暴露的时间依赖性反应，用三种不同表面电荷（1.2 µg mL）的 AgNPs 暴露人肝细胞（HepG2），并在 24 小时内监测完整的基因表达情况。时间和 AgNP 表面化学共同调控基因表达。此外，由于细胞在不断摄取营养，时间对基因表达有明显的影响，这一点应该考虑在内。AgNPs 的表面化学以时间依赖性的方式改变基因转录，其中阳离子 AgNPs 的影响最为显著。所有表面涂层都具有普遍性，AgNP 处理的细胞通过抑制增殖和激活细胞周期检查点来响应。通过使用配套的应用程序，可以更详细地分析 AgNP 细胞反应的这些普遍特征，以及特定的 AgNP 处理、时间点或特定基因。总之，这些结果为未来的风险评估提供了理解低剂量 AgNPs 对肝脏反应的基础。
