评价肉桂提取物对结肠癌患者分离的大肠杆菌 clbB 基因表达和生物膜形成的影响。
Evaluation of cinnamon extract effects on clbB gene expression and biofilm formation in Escherichia coli strains isolated from colon cancer patients.
机构信息
Microbiology Department, Faculty of Medicine, Hamadan University of Medical Sciences, P.O box: 6517838678, Hamadan, Iran.
Department of Community Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
BMC Cancer. 2020 Mar 30;20(1):267. doi: 10.1186/s12885-020-06736-1.
BACKGROUND
Colon cancer is one of the most common malignancies and the fourth leading cause of cancer-related mortality in the world. Colibactin, which is synthesized by the pks genomic island of E. coli interfere with the eukaryotic cell cycle. Cinnamon has an antimicrobial effect and considered as a colon cancer-preventing agent. The aim of the study was to evaluate the effects of cinnamon extract and cinnamaldehyde on clbB gene expression and biofilm formation in clinical isolates of E. coli.
METHODS
Thirty E. coli carrying pks gene were isolated from the colon cancer patients, inflammatory bowel disease and healthy subjects. Antibiotic susceptibility was evaluated by disk diffusion method and the minimum inhibitory concentration of cinnamon essential oil and cinnamaldehyde by microdilution broth method. In vitro biofilm formation of E.coli isolates was monitored using a microtiter plate method. The presence of clbB, clbA and clbQ genes in E.coli isolates were evaluated by PCR. The effect of cinnamaldehyde and cinnamon essential oil on clbB gene expression was evaluated by Real-Time PCR.
RESULTS
The highest antibiotic resistance was obtained with 94.4% for ticarcillin-clavulanic acid, azithromycin, amoxicillin, and amikacin. The MIC for all clinical isolates was 32 μl/ml of cinnamon essential oil and the MIC of cinnamaldehyde was between 0.00002 to 0.03 μl/ml. After exposure of isolates to cinnamon extract and cinnamaldehyde, 40 and 13.3% were weakly biofilm producers, respectively. The frequencies of clbB, clbA, and clbQ genes were 23.3, 23.3, and 26.7%, respectively. The expression of clbB gene in the presence of the Sub-MIC concentration of cinnamon essential oil and cinnamaldehyde was decreased in 8 isolates compared to untreated isolates (p-value < 0.05).
CONCLUSIONS
The antibacterial activity of cinnamaldehyde and cinnamon essential oil allows the use of these herbal compounds for treatment or supplements in infections caused by E. coli and in patients with suspected colorectal cancer.
背景
结肠癌是最常见的恶性肿瘤之一，也是世界上第四大癌症相关死亡原因。大肠杆菌的 pks 基因组岛合成的 colibactin 会干扰真核细胞周期。肉桂具有抗菌作用，被认为是预防结肠癌的药物。本研究旨在评估肉桂提取物和肉桂醛对临床分离的大肠杆菌 clbB 基因表达和生物膜形成的影响。
方法
从结肠癌患者、炎症性肠病患者和健康受试者的结肠中分离出 30 株携带 pks 基因的大肠杆菌。采用纸片扩散法评估抗生素敏感性，采用微量稀释肉汤法测定肉桂精油和肉桂醛的最小抑菌浓度。采用微量滴定板法监测大肠杆菌分离株的体外生物膜形成。通过 PCR 评估大肠杆菌分离株中 clbB、clbA 和 clbQ 基因的存在。通过实时 PCR 评估肉桂醛和肉桂精油对 clbB 基因表达的影响。
结果
对替卡西林-克拉维酸、阿奇霉素、阿莫西林和阿米卡星的耐药率最高，为 94.4%。所有临床分离株的 MIC 均为 32μl/ml 肉桂精油，肉桂醛的 MIC 介于 0.00002 至 0.03μl/ml 之间。在暴露于肉桂提取物和肉桂醛后，分别有 40%和 13.3%的分离株为弱生物膜生成者。clbB、clbA 和 clbQ 基因的频率分别为 23.3%、23.3%和 26.7%。与未处理的分离株相比，在肉桂精油和肉桂醛的亚 MIC 浓度下，8 株分离株 clbB 基因的表达降低（p 值<0.05）。
结论
肉桂醛和肉桂精油的抗菌活性允许将这些草药化合物用于治疗或补充由大肠杆菌引起的感染以及疑似结直肠癌的患者。
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