长链非编码 RNA MIR17HG 通过上调 miR-142-3p 抑制非小细胞肺癌来下调 Bach-1。
LncRNA MIR17HG inhibits non-small cell lung cancer by upregulating miR-142-3p to downregulate Bach-1.
机构信息
Department of Lung Cancer Surgery, General Hospital of Tianjin Medical University, No. 154 An'shan Road, Tianjin, 300052, China.
出版信息
BMC Pulm Med. 2020 Mar 30;20(1):78. doi: 10.1186/s12890-020-1112-3.
BACKGROUND
This study aimed to investigate the role of MIR17HG in non-small cell lung cancer (NSCLC).
METHODS
Differential expression of MIR17HG in NSCLC was first detected by exploring the TCGA dataset. Expression levels of miR-142-3p in both NSCLC and non-tumor tissues were determined by qPCR. The effects of overexpressing MIR17HG on the methylation of miR-142 were assessed by MSP. The effects of overexpressing MIR17HG, miR-142-3p and Bach-1 on the invasion and migration of NSCLC cells were assessed by Trasnwell invasion or migration assay.
RESULTS
Analysis of TCGA dataset revealed slightly downregulated expression of MIR17HG in NSCLC. This downregulation was further confirmed by measuring the expression levels of MIR17HG in NSCLC and non-tumor tissues from NSCLC patients. MIR17HG was found to decrease the methylation of miR-142-3p, and overexpression of MIR17HG led to upregulated miR-142-3p. Moreover, overexpression of MIR17HG also led to downregulated Bach-1, the downstream target of miR-142-3p. Cell invasion and migration analysis showed that overexpression of MIR17HG and miR-142-3p led to inhibited cancer cell invasion and migration. In contrast, overexpression of Bach-1 played an opposite role and attenuated the effects of overexpressing MIR17HG and miR-142-3p.
CONCLUSION
MIR17HG inhibits NSCLC by upregulating miR-142-3p to downregulate Bach-1.
TRIAL REGISTRATION
TJ-MU-2012-0148594, registered January 2, 2012.
背景
本研究旨在探讨 MIR17HG 在非小细胞肺癌（NSCLC）中的作用。
方法
首先通过探索 TCGA 数据集检测 NSCLC 中 MIR17HG 的差异表达。通过 qPCR 测定 NSCLC 和非肿瘤组织中 miR-142-3p 的表达水平。通过 MSP 评估过表达 MIR17HG 对 miR-142 的甲基化的影响。通过 Transwell 侵袭或迁移测定评估过表达 MIR17HG、miR-142-3p 和 Bach-1 对 NSCLC 细胞侵袭和迁移的影响。
结果
TCGA 数据集分析显示 NSCLC 中 MIR17HG 的表达略有下调。通过测量 NSCLC 患者的 NSCLC 和非肿瘤组织中 MIR17HG 的表达水平进一步证实了这一下调。发现 MIR17HG 降低了 miR-142-3p 的甲基化，过表达 MIR17HG 导致 miR-142-3p 上调。此外，过表达 MIR17HG 还导致下游靶基因 miR-142-3p 的 Bach-1 下调。细胞侵袭和迁移分析表明，过表达 MIR17HG 和 miR-142-3p 导致癌细胞侵袭和迁移受到抑制。相反，过表达 Bach-1 则起到相反的作用，减弱了过表达 MIR17HG 和 miR-142-3p 的作用。
结论
MIR17HG 通过上调 miR-142-3p 抑制 NSCLC，从而下调 Bach-1。
试验注册
TJ-MU-2012-0148594，于 2012 年 1 月 2 日注册。
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