Genome-based classification of three novel actinobacteria from the Karakum Desert: sp. nov., sp. nov. and sp. nov.

Three isolates, 5K138, 8K307 and KC603, with typical morphological characteristics of members of the genus were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliations of the strains. The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H) as a major menaquinone. Pairwise sequence comparisons of the 16S rRNA genes showed that the strains were closely related to DSM 45237, NEAU-YY265 and 3SM4-07 with higher than 99 % sequence identities. However, a combination of phenotypic and phylogenetic approaches as well as genome-based comparative analyses confirmed the taxonomic positions of these strains as representing distinct species within the genus . Therefore, strains 5K138, 8K307 and KC603 should each be classified as representing a novel species within the genus , for which the names sp. nov., sp. nov. and sp. nov. are proposed, respectively. The type strains of the proposed novel species are as follows: 5K138 (=JCM 33518=CGMCC 4.7672), 8K307 (=JCM 33519=CGMCC 4.7621) and KC603 (=JCM 33520=CGMCC 4.7618).