College of Biomass Science and Engineering, Sichuan University, Cheng Du 610065, China.
College of Biomass Science and Engineering, Sichuan University, Cheng Du 610065, China; Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, Sichuan University, Chengdu 610065, China.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110541. doi: 10.1016/j.msec.2019.110541. Epub 2019 Dec 12.
Nanofibrous drug delivery systems (DDSs) recently have attracted remarkable interest, especially their potential to program dosage of the encased drug intelligently. Despite this, the exploration of efficient strategy to precisely program drug release from nanofibrous DDS still remains a significant challenge. In this study, we electrospun a near-body temperature (T ≈ 42 °C) sensitive shape memory polyurethane in three stages through sequential electrospinning technology, and prepared a sort of sandwich structural membrane, comprising of top, inner and bottom layers, wherein a natural antibacterial agent, berberine hydrochloride (BCH), was imbedded inside the middle layer. As demonstrated by the results obtained from tensile testing and morphology characterization, the prepared sandwich structural membrane and the nanofibrous membrane with homogenous structure exhibited not only desirable mechanical properties but also surface morphologies. In addition, the release period can be significantly prolonged in virtue of the sandwich structure. As revealed by the experiment of in vitro drug release, it took nearly 144 h to release 80 wt% BCH from sandwich structural membrane, while as little as 72 h was observed to release the same amount of BCH from that with homogenous structure. More interestingly, the encapsulated BCH is capable to be released in a controlled manner owning to the thermo-sensitive shape memory effect, and the release rate of BCH can be accelerated by stretching and fixing the nanofibrous membranes into certain ratios prior to release. Collectively, this study provides a facile strategy to design and prepare a reliable and smart DDS, i.e. sandwich structural membrane, which may enhance the availability of BCH and also intelligently avoid the bacterial infection.
纳米纤维药物输送系统 (DDS) 最近引起了人们的极大兴趣，尤其是其智能控制包裹药物剂量的潜力。尽管如此，探索从纳米纤维 DDS 中精确控制药物释放的有效策略仍然是一个重大挑战。在这项研究中，我们通过顺序静电纺丝技术，在三个阶段共纺制了一种接近体温（T≈42°C）敏感的形状记忆型聚氨酯，并制备了一种三明治结构膜，包括顶层、内层和底层，其中盐酸小檗碱（BCH）被嵌入到中间层中。由拉伸试验和形貌表征结果可知，所制备的三明治结构膜和具有均匀结构的纳米纤维膜不仅具有理想的力学性能，而且具有良好的表面形貌。此外，由于三明治结构的存在，释放周期可以显著延长。体外药物释放实验表明，从三明治结构膜中释放 80wt%的 BCH 大约需要 144 小时，而从具有均匀结构的膜中释放相同量的 BCH 只需要 72 小时。更有趣的是，由于热敏感形状记忆效应，包裹的 BCH 能够以受控的方式释放，并且在释放之前将纳米纤维膜拉伸并固定成一定比例可以加速 BCH 的释放速度。总之，本研究提供了一种简单的设计和制备可靠智能 DDS 的策略，即三明治结构膜，这可能提高 BCH 的可用性，并智能地避免细菌感染。
