CMEMS-UMinho, Center for MicroElectroMechanical Systems of the University of Minho, 4800-058 Guimarães, Portugal; TEMA-Centre for Mechanical Technology and Automation, Department of Mechanical Engineering, University of Aveiro, Campus de Santiago, Aveiro 3810-193, Portugal.
Institute of Biomaterials, Department of Materials Science and Engineering, University of Erlangen-Nuremberg, Erlangen, 91058, Germany.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110492. doi: 10.1016/j.msec.2019.110492. Epub 2019 Dec 12.
Laser texturing is a technique that has been increasingly explored for the surface modification of several materials on different applications. Laser texturing can be combined with conventional coating techniques to functionalize surfaces with bioactive properties, stimulating cell differentiation and adhesion. This study focuses on the cell adhesion of laser-sintered coatings of hydroxyapatite (HAp) and 45S5 bioactive glass (45S5 BG) on zirconia textured surfaces using MC3T3-E1 cells. For this purpose, zirconia surfaces were micro-textured via laser and then coated with HAp and 45S5 BG glass via dip coating. Afterwards, the bioactive coatings were laser sintered, and a reference group of samples was conventionally sintering. The cell adhesion characterisation was achieved by cell viability performing live/dead analysis using fluorescence stains and by SEM observations for a qualitative analysis of cell adhesion. The in vitro results showed that a squared textured pattern with 100μm width grooves functionalized with a bioactive coating presented an increase of 90% of cell viability compared to flat surfaces after 48h of incubation. The functionalized laser sintered coatings do not present significant differences in cell viability when compared to conventionally sintered coatings. Therefore, the results reveal that laser sintering of HAp and 45S5 BG coatings is a fast and attractive coating technique.
激光纹理处理是一种越来越受到关注的技术，可用于对不同应用中多种材料的表面进行改性。激光纹理处理可以与传统的涂层技术结合使用，为具有生物活性的表面赋予功能性，从而刺激细胞分化和黏附。本研究使用 MC3T3-E1 细胞，聚焦于激光烧结的羟基磷灰石（HAp）和 45S5 生物活性玻璃（45S5 BG）涂层在经过微纹理处理的氧化锆表面上的细胞黏附。为此，通过激光对氧化锆表面进行微纹理处理，然后通过浸涂在其表面涂覆 HAp 和 45S5 BG 玻璃。之后，对生物活性涂层进行激光烧结，并对参考组样品进行常规烧结。通过荧光染色进行活/死分析来实现细胞黏附特性的评估，并通过 SEM 观察进行细胞黏附的定性分析。体外结果表明，经过 48 小时孵育后，与平面相比，具有 100μm 宽凹槽的方形纹理图案功能化的生物活性涂层的细胞活力增加了 90%。与常规烧结涂层相比，激光烧结的生物活性涂层在细胞活力方面没有显著差异。因此，研究结果表明，激光烧结 HAp 和 45S5 BG 涂层是一种快速且有吸引力的涂层技术。
