Department of Chemistry, Fordham University, 441 East Fordham Road, Bronx, NY 10458, United States of America.
Department of Chemistry, Fordham University, 441 East Fordham Road, Bronx, NY 10458, United States of America.
Mater Sci Eng C Mater Biol Appl. 2020 Apr;109:110540. doi: 10.1016/j.msec.2019.110540. Epub 2019 Dec 20.
New peptide based hybrid scaffolds were prepared by blending two different fish scale derived hydroxyapatite with functionalized peptide nanofibers for potential applications in periodontal tissue regeneration. The nanofibers were prepared by self-assembly of the newly designed peptide bolaamphiphile Bis (N-α-amido-glutamic acid) 1,7 heptane tetracarboxylate and functionalized with a segment of the tyrosine rich amylogenin peptide sequence MPLPPHPGHPGYINF followed by polygalacturnonic acid and hydroxyapatite derived from salmon or red-snapper fish scales. The binding interactions of the components of the scaffold was confirmed by FTIR spectroscopy as well as SEM imaging. Hybrids scaffolds with salmon scale derived HaP showed higher mechanical strength and Young's Modulus compared to snapper scale derived scaffolds. Our results indicated that while both the scaffolds supported cell proliferation and efficiently formed cell-scaffold matrices with gingival fibroblasts, we observed greater alignment of the cells in the case of scaffolds that contained snapper scale derived hydroxyapatite. Furthermore, higher differentiation ability into osteoblast like cells was seen in the case of the snapper scale derived HaP based scaffolds. Our studies indicate that the hybrid peptide nanofiber scaffold matrices, particularly those prepared using snapper scales may have significant utility in the development of biomaterials for periodontal tissue regeneration.
新型基于肽的杂化支架通过混合两种不同的鱼鳞衍生羟基磷灰石与功能化肽纳米纤维来制备，用于牙周组织再生的潜在应用。纳米纤维通过新设计的肽双（N-α-酰胺-谷氨酸）1,7-庚烷四羧酸的自组装制备，并与富含酪氨酸的藻氨酸肽序列 MPLPPHPGHPGYINF 的一段进行功能化，随后与源自三文鱼或红鲷鱼鳞的聚半乳糖醛酸和羟基磷灰石结合。支架组件的结合相互作用通过傅里叶变换红外光谱和 SEM 成像得到证实。与源自红鲷鱼鳞的支架相比，源自三文鱼鱼鳞的 HaP 杂化支架表现出更高的机械强度和杨氏模量。我们的结果表明，虽然两种支架都支持细胞增殖，并有效地与牙龈成纤维细胞形成细胞-支架基质，但我们观察到在含有源自红鲷鱼鳞的羟基磷灰石的支架中，细胞的排列更为整齐。此外，源自红鲷鱼鳞的 HaP 支架的成骨细胞样细胞的分化能力更高。我们的研究表明，杂化肽纳米纤维支架基质，特别是使用红鲷鱼鳞制备的那些，可能在牙周组织再生生物材料的开发中具有重要的应用价值。
