Hao Dan, Wang Xiao, Thomsen Bo, Kadarmideen Haja N, Wang Xiaogang, Lan Xianyong, Huang Yongzhen, Qi Xinglei, Chen Hong
College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Animal Genetics, Breeding and Reproduction, Yangling 712100, Shaanxi, China.
Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus C, Denmark.
Animals (Basel). 2020 Mar 27;10(4):566. doi: 10.3390/ani10040566.
Association studies have indicated profound effects of copy number variations (CNVs) on various phenotypes in different species. In this study, we identified the CNV distributions and expression levels of guanylate-binding protein 6 () associated with the growth traits of Chinese cattle. The results showed that the phenotypic values of body size and weight of Xianan (XN) cattle were higher than those of Nanyang (NY) cattle. The medium CNV types were mostly identified in the XN and NY breeds, but their CNV distributions were significantly different (adjusted < 0.05). The association analysis revealed that the body weight, cannon circumference and chest circumference of XN cattle had significantly different values in different CNV types ( < 0.05), with CNV gain types (Log2 > 0.5) displaying superior phenotypic values. We also found that transcription levels varied in different tissues ( < 0.001) and the CNV gain types showed the highest relative gene expression levels in the muscle tissue, consistent with the highest phenotypic values of body weight and cannon circumference among the three CNV types. Consequently, our results suggested that CNV gain types of could be used as the candidate markers in the cattle-breeding program for growth traits.
关联研究表明，拷贝数变异（CNV）对不同物种的各种表型具有深远影响。在本研究中，我们鉴定了与中国黄牛生长性状相关的鸟苷酸结合蛋白6（）的CNV分布和表达水平。结果表明，夏南牛（XN）的体尺和体重表型值高于南阳牛（NY）。中等CNV类型大多在XN和NY品种中鉴定到，但其CNV分布存在显著差异（校正后<0.05）。关联分析显示，XN牛的体重、管围和胸围在不同CNV类型中具有显著不同的值（<0.05），CNV增加型（Log2>0.5）表现出更优的表型值。我们还发现，转录水平在不同组织中存在差异（<0.001），且CNV增加型在肌肉组织中显示出最高的相对基因表达水平，这与三种CNV类型中体重和管围的最高表型值一致。因此，我们的结果表明，的CNV增加型可作为牛生长性状育种计划中的候选标记。
