Department of Pathogenic Microbiology & Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, 76 West Yanta Road, Xi'an, 710061, China.
The Second Hospital of Weinan, 2 East Chaoyang Street, Weinan, 714000, China.
Photochem Photobiol Sci. 2020 Apr 15;19(4):485-494. doi: 10.1039/c9pp00352e.
The extensive and repetitive use of antifungal drugs has led to the development of drug-resistant Candida albicans. Antimicrobial photodynamic therapy (aPDT) has received considerable attention as an emerging and promising approach to combat drug-resistant microbes. This study evaluated the photodynamic effects mediated by aloe emodin (AE), a natural compound isolated from Aloe vera and Rheum palmatum, on azole-sensitive and azole-resistant C. albicans in vitro. AE exhibited no significant dark toxicity, but in the presence of light, effectively inactivated C. albicans cells in a concentration-dependent manner. The uptake of AE by fungal cells was investigated by confocal laser scanning microscopy (CLSM), and the results showed that AE possessed stronger ability to enter into C. albicans cells following light irradiation. Transmission electron microscopy analysis suggested that AE-mediated aPDT could induce damage to the cell wall, cytoplasm, and nucleus. Damage to the surface of C. albicans was observed by scanning electron microscopy. These results suggest that AE is a potential PS for use in aPDT of drug-resistant C. albicans strains, and AE-mediated aPDT shows promise as an antifungal treatment.
抗真菌药物的广泛和重复使用导致了耐药性白色念珠菌的出现。光动力抗菌疗法（aPDT）作为一种新兴的、有前途的方法来对抗耐药微生物，受到了广泛关注。本研究评估了从库拉索芦荟和大黄中分离得到的天然化合物大黄素（AE）对体外唑类敏感和唑类耐药白色念珠菌的光动力作用。AE 在黑暗中没有表现出明显的毒性，但在光照下，以浓度依赖的方式有效地灭活白色念珠菌细胞。通过共聚焦激光扫描显微镜（CLSM）研究了真菌细胞对 AE 的摄取，结果表明，AE 在光照射后具有更强的进入白色念珠菌细胞的能力。透射电子显微镜分析表明，AE 介导的 aPDT 可诱导细胞壁、细胞质和细胞核损伤。扫描电子显微镜观察到白色念珠菌表面受损。这些结果表明，AE 是一种用于治疗耐药白色念珠菌菌株的潜在 PS，AE 介导的 aPDT 有望成为一种抗真菌治疗方法。
