Li Jiaxin, Ling Mengmeng, Sun Yixue, Di Haiyang, Cong Yulin, Yu Haiying, Cong Yanlong
Laboratory of Infectious Diseases, College of Veterinary Medicine, Key Laboratory of Zoonosis Research, Ministry of Education, Jilin University, Changchun 130062, China.
Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun 130062, China.
J Vet Sci. 2020 Mar;21(2):e19. doi: 10.4142/jvs.2020.21.e19.
Given that the current Newcastle disease virus (NDV) infection in wild birds poses the threat to poultry, surveillance of Newcastle disease in captive wild birds was carried out in Jilin, China in 2018. Here, an NDV strain obtained from toco toucan was firstly characterized. The results showed that the F gene of the NDV isolate Toucan/China/3/2018 is classified as genotype II in class II. Sequence analysis of the F0 cleavage site was RQGR/L, which supports the result of the intracerebral pathogenicity index assay indicating classification of the isolate as low-pathogenicity. Experimental infection demonstrated that Toucan/China/3/2018 can effectively replicate and transmit among chickens. To our knowledge, this is the first report on genetically and pathogenically characterizing NDV strain isolated from toucan, which enriches the epidemiological information of NDV in wild birds.
鉴于目前野生鸟类中的新城疫病毒（NDV）感染对家禽构成威胁，2018年在中国吉林对圈养野生鸟类进行了新城疫监测。在此，首次对从托哥巨嘴鸟获得的一株NDV毒株进行了特征分析。结果表明，NDV分离株Toucan/China/3/2018的F基因在第二类中被归类为第二基因型。F0裂解位点的序列分析结果为RQGR/L，这支持了脑内致病性指数测定结果，表明该分离株为低致病性。实验感染表明，Toucan/China/3/2018能够在鸡之间有效复制和传播。据我们所知，这是关于从巨嘴鸟分离的NDV毒株的遗传和致病性特征分析的首次报告，丰富了野生鸟类中NDV的流行病学信息。
