College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Daqing, 163319, China.
Key Laboratory of Zoonosis Research, Ministry of Education/Institute of Zoonosis, Jilin University, Changchun, 130062, China.
Pol J Vet Sci. 2020 Mar;23(1):59-67. doi: 10.24425/pjvs.2020.132749.
Anestrus is essential to an unsuccessful pregnancy in dairy cows. One of the many factors that influences anestrus is the inactive ovary. To characterize in detail the plasma metabolic pro- file, anestrus cows suffering from inactive ovaries were compared with those with natural estrus. The Holstein cows 60 to 90 day postpartum in an intensive dairy farm were assigned into inactive ovaries groups (IO, n=20) and natural estrus group (CON, n=22) according to estrus signs and rectal palpation of ovaries. Plasma samples from two groups of cows were collected from the tail vein to screen differential metabolites using gas chromatography/mass spectrometry (GC/MS) techniques and multivariate statistical analysis and pathways. The results showed that 106 compounds were screened by GC/MS and 14 compounds in the IO group were decreased by analyzing important variables in the projection values and p values of MSA.Through pathway analysis, 14 compounds, mainly associated with carbohydrate metabolism and amino acid meta- bolism, were identified to results in IO, which may seriously affect follicular growth. Metabolo- mics profiling, together with MSA and pathway analysis, showed that follicular growth and development in dairy cows is related to carbohydrate and amino acid metabolism by a single or multiple pathway(s).
乏情是奶牛妊娠失败的关键因素之一。影响乏情的众多因素之一是卵巢静止。为了详细描述血浆代谢特征，本研究比较了患有卵巢静止的乏情奶牛和具有自然发情的奶牛。从集约化奶牛场产后 60-90 天的荷斯坦奶牛中，根据发情迹象和直肠触诊卵巢，将其分为卵巢静止组（IO，n=20）和自然发情组（CON，n=22）。从两组奶牛的尾静脉采集血浆样本，使用气相色谱/质谱（GC/MS）技术和多变量统计分析及途径对差异代谢物进行筛选。结果表明，GC/MS 筛选出 106 种化合物，通过分析 MSA 投影值和 p 值中的重要变量，发现 IO 组中有 14 种化合物减少。通过途径分析，确定了 14 种与碳水化合物代谢和氨基酸代谢密切相关的化合物，这些化合物可能严重影响卵泡的生长。代谢组学分析与 MSA 和途径分析表明，奶牛的卵泡生长和发育与单一或多种途径（s）的碳水化合物和氨基酸代谢有关。
