College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, Heilongjiang 163319, China.
Heilongjiang Provincial Technology Innovation Center for Bovine Disease Control and Prevention, Daqing 163319, China.
Biomed Res Int. 2020 Nov 16;2020:9837543. doi: 10.1155/2020/9837543. eCollection 2020.
The metabolism of dairy cows with inactive ovaries differs from that of healthy dairy cows. However, the molecular mechanisms underpinning these physiological and metabolic changes remain unclear. The purpose of this study was to investigate follicular fluid metabolite changes in dairy cows with inactive ovaries. Untargeted metabolomics technology and multivariate statistical analysis were used to screen differential metabolites in follicular fluid samples between inactive ovaries and estrus cows at 45-60 d postpartum. Fourteen differential metabolites were identified, consisting of amino acids, lipids, sugars, and nucleotides. When compared with healthy animal samples, eight follicular fluid metabolites were significantly increased, and six metabolites were significantly decreased in dairy cows with inactive ovaries. Metabolic pathway analyses indicated that differential metabolites were primarily involved in glycerol phospholipid metabolism, arachidonic acid metabolism, valine, leucine and isoleucine biosynthesis, and phenylalanine metabolism. These metabolites and their enrichment pathways indicate that the enhancement of lipid metabolism and the weakening of carbohydrate production of amino acids in dairy cows with impaired follicular development. Overall, these data provide a better understanding of the changes that could affect follicular development during the postpartum period and lay the ground for further investigations.
不活跃卵巢奶牛的代谢与健康奶牛的代谢不同。然而，这些生理和代谢变化的潜在分子机制尚不清楚。本研究旨在研究不活跃卵巢奶牛的卵泡液代谢产物变化。使用非靶向代谢组学技术和多变量统计分析筛选产后 45-60 天不活跃卵巢和发情奶牛的卵泡液样本中的差异代谢物。鉴定出 14 种差异代谢物，包括氨基酸、脂类、糖和核苷酸。与健康动物样本相比，不活跃卵巢奶牛的卵泡液中 8 种代谢物显著增加，6 种代谢物显著减少。代谢途径分析表明，差异代谢物主要涉及甘油磷脂代谢、花生四烯酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成以及苯丙氨酸代谢。这些代谢物及其富集途径表明，在受损卵泡发育的奶牛中，脂质代谢增强，氨基酸的碳水化合物生成减弱。总体而言，这些数据更好地理解了产后期间可能影响卵泡发育的变化，并为进一步的研究奠定了基础。
