Department of Hand Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P. R. China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun, Jilin 130012, P. R. China.
ACS Chem Neurosci. 2020 May 6;11(9):1359-1370. doi: 10.1021/acschemneuro.0c00116. Epub 2020 Apr 13.
Peripheral nerve injuries always cause dysfunction but without ideal strategies to assist the treatment and recovery successfully. The primary way to repair the peripheral nerve injuries is to bridge the lesions by promoting axon regeneration. Schwann cells acting as neuroglial cells play a pivotal role during axonal regeneration. The orderly and organized migration of Schwann cells is beneficial for the extracellular matrix connection and Büngner bands formation, which greatly promote the regeneration of axons by offering mechanical support and growth factors. Thus, the use of Schwann cells as therapeutic cells offers us an attractive method for neurorepair therapies, and the ability to direct and manipulate Schwann cell migration and distribution is of great significance in the field of cell therapy in regards to the repair and regeneration of the peripheral nerve. Herein, we design and characterize a type of novel fluorescent-magnetic bifunctional FeO·Rhodamine 6G (R6G)@polydopamine (PDA) superparticles (SPs) and systematically study the biological behaviors of FeO·R6G@PDA SP uptake by Schwann cells. The results demonstrate that our tailor-made FeO·R6G@PDA SPs can be endocytosed by Schwann cells and then highly magnetize Schwann cells by virtue of their excellent biocompatibility. Furthermore, remote-controlling and noninvasive magnetic targeting migration of Schwann cells can be achieved on the basis of the high magnetic responsiveness of FeO·R6G@PDA SPs. At the end, gene expression profile analysis is performed to explore the mechanism of Schwann cells' magnetic targeting migration. The results indicate that cells can sense external magnetic mechanical forces and transduce into intracellular biochemical signaling, which stimulate gene expression associated with Schwann cell migration.
周围神经损伤总是导致功能障碍，但目前没有理想的策略来成功辅助治疗和恢复。修复周围神经损伤的主要方法是通过促进轴突再生来桥接损伤。施万细胞作为神经胶质细胞，在轴突再生过程中发挥着关键作用。施万细胞的有序和组织化迁移有利于细胞外基质的连接和 Büngner 带的形成，这极大地促进了轴突的再生，为其提供了机械支持和生长因子。因此，将施万细胞作为治疗细胞为神经修复治疗提供了一种有吸引力的方法，而指导和操纵施万细胞迁移和分布的能力在细胞治疗领域对于周围神经的修复和再生具有重要意义。在这里，我们设计并表征了一种新型荧光-磁性双功能 FeO·Rhodamine 6G（R6G）@聚多巴胺（PDA）超粒子（SPs），并系统研究了 FeO·R6G@PDA SP 被施万细胞摄取的生物学行为。结果表明，我们定制的 FeO·R6G@PDA SPs 可以被施万细胞内吞，然后由于其优异的生物相容性而使施万细胞高度磁化。此外，基于 FeO·R6G@PDA SPs 的高磁响应性，可以实现对施万细胞的远程控制和非侵入性磁靶向迁移。最后，进行基因表达谱分析以探讨施万细胞磁靶向迁移的机制。结果表明，细胞可以感知外部磁机械力，并将其转化为细胞内生化信号，从而刺激与施万细胞迁移相关的基因表达。
