Sharifi Majid, Salehi Majid, Ebrahimi-Barough Somayeh, Alizadeh Morteza, Jahromi Hossein Kargar, Kamalabadi-Farahani Mohammad
Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
J Nanobiotechnology. 2024 Dec 19;22(1):776. doi: 10.1186/s12951-024-03048-5.
Numerous conduits have been developed to improve peripheral nerve regeneration. However, challenges remain, including remote control of conduit function, and programmed cell behaviors like orientation. We synthesized FeO-MnO@Zirconium-based Metal-organic frameworks@Retinoic acid (FMZMR) core-shell and assessed their impact on Schwann cell function and behavior within conduits made from decellularized human umbilical arteries (DHUCA) under magnetic field (MF). FMZMR core-shell, featuring a spherical porous structure and catalytic properties, effectively scavenges radicals and facilitates controlled drug release under MF. The histology of the DHUCA indicates effective decellularization with adequate tensile strength and Young's modulus for sciatic nerve regeneration. In-vitro results demonstrate that FMZMR core-shell is biocompatible and promotes Schwann cell proliferation through remotely controlled drug release. Furthermore, its synergy with MF enhances cell orientation and increases neurite length by ~ 1.93-fold. Functional and histological evaluations indicate that the FMZMR core-shell combined with MF promotes nerve regeneration, decreases muscle atrophy, and enhances new neuron growth and myelin formation, without negatively affecting vital tissues. This study suggests that the synergistic effect of FMZMR core-shell with MF can alleviate some of the treatment challenges.
人们已经开发出多种导管来促进周围神经再生。然而，挑战依然存在，包括对导管功能的远程控制以及诸如定向等程序性细胞行为。我们合成了FeO-MnO@锆基金属有机框架@视黄酸（FMZMR）核壳结构，并评估了它们在磁场（MF）作用下对由脱细胞人脐动脉（DHUCA）制成的导管内雪旺细胞功能和行为的影响。具有球形多孔结构和催化特性的FMZMR核壳结构能有效清除自由基，并在磁场作用下促进药物的可控释放。DHUCA的组织学检查表明其脱细胞效果良好，具有足够的拉伸强度和杨氏模量以促进坐骨神经再生。体外实验结果表明，FMZMR核壳结构具有生物相容性，并通过远程控制药物释放促进雪旺细胞增殖。此外，它与磁场的协同作用增强了细胞定向，并使神经突长度增加了约1.93倍。功能和组织学评估表明，FMZMR核壳结构与磁场相结合可促进神经再生，减少肌肉萎缩，并增强新神经元生长和髓鞘形成，而不会对重要组织产生负面影响。这项研究表明，FMZMR核壳结构与磁场的协同效应可以缓解一些治疗挑战。
